Design and in vitro evaluation of delivery systems for co-delivery of siRNA and proteins by mimetic exosomes
10.16438/j.0513-4870.2019-0590
- VernacularTitle:人工外泌体共递送siRNA和蛋白的递送系统的设计及体外评价
- Author:
Sai DENG
1
;
Ling-min ZHANG
1
;
Ping WANG
1
;
Shi-ying LI
1
;
Chao-jin LIN
1
;
Xiao-mei FU
1
;
Xi-yong YU
1
Author Information
1. Guangdong Key Laboratory of Molecular Target & Clinical Pharmacology and the State Key Laboratory of Respiratory Disease Pharmacology Group, Guangzhou Medical University, Guangzhou 511436, China
- Publication Type:Research Article
- Keywords:
mimetic exosome;
polylactic acid-glycolic acid copolymer;
liposome;
nano-particles;
rug delivery
- From:
Acta Pharmaceutica Sinica
2020;55(1):139-145
- CountryChina
- Language:Chinese
-
Abstract:
To prepare the mimetic exosomes and co-delivery proteins and nucleic acids, and achieve efficient and safe co-delivery of multi-component drugs, an optimized formulation was designed by modifying a polylactic acid-glycolic acid copolymer (PLGA) matrix with a cationic lipid excipient dioleyl trimethylammonium propane (DOTAP), and a PLGA/DOTAP nanoparticles packaged protein and nucleic acid was prepared by double emulsion method, and the outermost membrane structure prepared by reverse phase evaporation method and consists of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), cholesterol and membrane proteins. The structure of the mimetic exosomes is formed by ultrasonic dispersion and extrusion, and analyzed its characteristics and nature of the transfer effect. The size of mimetic exosomes was about 156.13 nm, with negative charge (-18.23 ± 0.57 mV), and it could efficiently co-transfer protein and siRNA, and siRNA could effectively inhibit the expression of target gene Trim28. The mimetic exosomes simulate the structure of exosomes and achieve safe and efficient co-delivery of multi-component drugs.
