Qualitative screening of 169 veterinary drug residues in bearbile powder by QuEChERS-ultra high performance liquid chromatography-tandem mass spectrometry
10.16438/j.0513-4870.2019-0531
- VernacularTitle:QuEChERS-超高效液相色谱串联质谱法定性筛查中药材熊胆粉中169种兽药残留
- Author:
Jian SUN
1
;
Qing HU
1
;
Yan-rong ZHU
1
;
Su ZHANG
1
;
Hong YU
1
;
Rui FENG
1
;
Jing-xian ZHANG
1
;
Xiu-hong MAO
1
;
Shen JI
1
Author Information
1. NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine, Shanghai Institute for Food and Drug Control, Shanghai 201203, China
- Publication Type:Research Article
- Keywords:
QuEChERS;
ultra high performance liquid chromatography-tandem mass spectrometry;
veterinary drug;
bear bile powder;
qualitative screening
- From:
Acta Pharmaceutica Sinica
2020;55(1):113-122
- CountryChina
- Language:Chinese
-
Abstract:
A QuEChERS-ultra high performance liquid chromatography-tandem mass spectrometry method was developed for qualitative screening of 169 veterinary drug residues in bear bile powder, including β-agonists and inhibitors, antibiotics (penicillins, β-lactams, sulfomamides, quinolones, chloramphenicals, tetracyclines, nitroimidazoles, macrolides, polyethers, etc.), antiviral drugs, anthelminitics, steroid hormones, nonsteroidal antiinflammatory drugs (NSAIDs) and sedatives. The samples were extracted by Na2EDTA-McIlvaine buffer solution and 5% fomic acid-acetonitrile solution, then purified by dispersive solid phase extraction. Detection of veterinary drug residues by ultra high performance liquid chromatography-triple quadrupole mass spectrometry was conducted and qualitative confirmed by ion ratios. The limits of detection of 169 veterinary drugs were 1-1 000 μg·kg-1. The method is simple and fast, which had been used for the analysis of actual samples, and can be extended to the detection of similar matrix.
