Comparison of gel-aided sample preparation (GASP) and two in-solution digestion workflows for proteomic analysis using Saccharomyces cerevisiae lysate

Author: Nashriq Mohammad Jailani ¹ ; Asmahani Azira Abdu Sani ² ; Roman Fischer ³ ; Honglei Huang ³ ; Wan Mohd Aizat ¹ ; Sheila Nathan ¹ ; Holger B. Kramer ⁴ ; Benedikt M. Kessler ³ ; Mukram Mohamed Mackeen ¹
Author Information

1. Universiti Kebangsaan Malaysia
2. Malaysia Genome Institute,
3. University of Oxford
4. Hammersmith Hospital Campus
Publication Type:Journal Article
Keywords: GASP; in-solution digestion; proteomic analysis; Saccharomyces cerevisiae
From:Malaysian Journal of Microbiology 2018;14(6):579-584
CountryMalaysia
Language:English
Abstract: Abstracts:Using Saccharomyces cerevisiae lysate, two in-solution trypsin digestions (chloroform-methanol-water precipitation and RapiGest) were compared to the recently reported gel-aided sample preparation (GASP) workflow. Our proteomic results showed that GASP afforded the highest number of overall protein identifications and peptide spectrum matches without systematic bias towards peptide or protein size.
Full text:20.2018my0491.pdf