Primary culture of hPDSCs with modified enzymatic digestion-explants method
10.12016/j.issn.2096⁃1456.2018.03.005
- Author:
LIU Ying
1
;
GAO Jie
2
;
WU Buling
3
Author Information
1. Stomatological Hospital, Southern Medical University
2. Department of Stomatology, Guangzhou Medical University
3. Nanfang Hospital, Southern Medical University & Stomatological College of Southern Medical University
- Publication Type:Journal Article
- Keywords:
hDPSCs;
Explants method and enzymatic separation method;
Primary culture;
Cells identification
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2018;26(3):166-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore methods using modified tissue enzymatic separations for culturing primary hDPSCs in vitro and further identify the cells produced.
Methods :Primary hDPSCs were cultured using the modified tissue enzymatic separation method, and cells were identified by morphology, cell surface markers, and differentiation potential and evaluated using flow cytometry and growth curves.
Results :The hDPSCs were successfully isolated using the modified tissue enzymatic separation method. The morphology of these cells was similar to that of fibroblasts and mesenchymal stem cells, and the growth curve was "S" -type. The results of cell phenotype analysis indicated that the cells were positive for surface markers of mesenchymal stem cells, including CD29, CD44, and CD90, and negative for markers of hematopoietic stem cells, including CD34, CD45, and CD106. The cells were capable of differentiating into multiple cell types.
Conclusion : The modified tissue enzymatic separation method can successful be used to culture primary hDPSCs in vitro.
- Full text:改良组织块酶消化法原代培养人牙髓干细胞的研究.pdf
