Diversity of microbiota composition of traditional Chinese medicine of Faeces Trogopterori from Shangluo, Shanxi province by sequence of the bacterial 16S rRNA gene
10.16438/j.0513-4870.2019-0517
- VernacularTitle:基于16S rRNA基因测序的陕西商洛区五灵脂菌群结构分析
- Author:
Xiao-song HU
1
;
Dan JIANG
1
;
Ting LI
1
;
Zhen XU
1
;
Jing-ge LIU
2
;
Chun-sheng LIU
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
2. Shang Fang Tang Traditional Chinese Medicine Limited Company, Lanzhou 730030, China
- Publication Type:Research Article
- Keywords:
Faeces Trogopterori;
flora structure;
16sRNA sequencing;
functional prediction;
fecal transplantation
- From:
Acta Pharmaceutica Sinica
2019;54(12):2335-2339
- CountryChina
- Language:Chinese
-
Abstract:
To obtain the microbial composition of traditional Chinese medicine of Faeces Trogopterori, ten samples were collected from the imitate wildness farmland in Shangluo City, Shaanxi Province. In this study, 16S rRNA gene was used as molecular marker to explore the microbiome and the sequences were analyzed by Usearch analysis platform. The COG and KEGG database is used to predict and analyze the function of the flora. A great number of 285 218 high quality clean reads with a length of 400-450 bp were obtained from 10 samples. Bacterial species detected in these samples covered 8 phyla, 25 families, 75 genera and 120 species. The dominant phylum microbial communities in these samples were Firmicutes (87.68% ± 2.68%) and the Bacteroidetes (7.62% ± 3.74%), all samples showed a high microbial diversity, the predicted functional metagenome was heavily involved in energy metabolism. This study provided that the beneficial bacteria in Faeces Trogopterori may be one of its active ingredients, and no pathogens are detected in the sample.
