Quality ecotype of Panax quinquefolium L. based on heredity-chemistry-ecology characteristics
10.16438/j.0513-4870.2019-0394
- VernacularTitle:基于遗传-化学-生态特征的西洋参品质生态型研究
- Author:
Xiao SUN
1
;
Qiu-yu QIAN
2
;
Si-hao ZHENG
1
;
Hai-mei CHEN
3
;
Lin-fang HUANG
1
Author Information
1. Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China; Engineering Research Center of Chinese Medicine Resource, Ministry of Education, Beijing 100193, China
2. The 263nd Clinical Department, General Hospital of Beijing Military Area Command of PLA, Beijing 101149, China
3. Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
- Publication Type:Research Article
- Keywords:
italic>Panax quinquefolium L.;
quality ecology on chinese medicines;
transcriptome;
ginsenoside;
aodi herbs
- From:
Acta Pharmaceutica Sinica
2019;54(9):1695-1705
- CountryChina
- Language:Chinese
-
Abstract:
Medicinal materials in China differ in quality by different ecological types. Our research group found that there were two ecotypes of domestic Panax quinquefolium L. according to the characteristics of ginsenosides, inside versus outside Shanhaiguan. The genetic and ecological mechanisms of quality variation of Panax quinquefolium L. is unknown. Based on the genetic-chemical-ecological strategy, transcriptome and HPLC technology were used for comprehensive correlation analyses of transcriptomic data, ginsenoside content and environmental climate ecological factors. The transcriptomic results showed that key genes of ginsenoside biosynthesis, such as HMGR, AS and FPS, were significantly down-regulated in the inside Shanhaiguan ecotype. HPLC results showed that the quality of outside Shanhaiguan ecotype Panax quinquefolium L. was higher than that of the inside ecotype, with the content of ginsenosides in outside Panax quinquefolium L. was higher than that of inside ecotype except Rb2. Correlation analyses revealed that content of Panax quinquefolium L. ginsenoside is positively related to the expression levels of ginsenoside biosynthesis key genes (MK, HMGS, HMGR, and AS), and negatively related to the expression of glycosyl transferase (GT). The content of ginsenosides is negative related with climate factors, such as temperature, sunshine, and is positively related with moisture in both ecological environments. This study has provided a new mechanistic insight into the quality variations of two ecotypes for Panax quinquefolium L. and established a scientific basis for studying the ecological factors for the quality of traditional Chinese medicine.
