Application of Mycobacterium marinum infected zebrafish for evaluation of anti-tuberculosis drugs
10.16438/j.0513-4870.2018-1110
- VernacularTitle:基于海分枝杆菌感染斑马鱼模型的抗结核药物评价方法研究
- Author:
Guo-wei YANG
1
;
Shi-ze PENG
2
;
Jian-yuan ZHAO
2
;
Yong-xin ZHANG
2
;
Shan CEN
2
Author Information
1. Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing 100050, China
2. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
- Publication Type:Research Article
- Keywords:
italic>Mycobacterium marinum;
anti-tuberculosis drug;
zebrafish model;
rug evaluation;
qPCR
- From:
Acta Pharmaceutica Sinica
2019;54(5):854-860
- CountryChina
- Language:Chinese
-
Abstract:
Tuberculosis (TB) is a serious infectious disease caused by Mycobacterium. tuberculosis. In recent years, with the emergence of drug-resistant forms, the development of new anti-tuberculosis drugs is urgently needed. In this study, we used Mycobacterium marinum (M. marinum), which is highly similar to M. tuberculosis, to establish a M. marinum infected-zebrafish model and quantitative PCR (qPCR) method for bacterial count analysis. The results showed that injecting M. marinum into the yolk sac is an efficient and convenient way to infect zebrafish embryos. By counting the survival rate of infected zebrafish and the number of bacteria in zebrafish by Ziehl-Neelsen staining, we analyzed the efficacy of isoniazid and rifampicin as anti-tuberculosis drugs and the synergistic effect of drugs. The results suggested that three evaluation methods exhibit good consistency. This study demonstrated that zebrafish-M. marinum infection model combined with qPCR analysis is a simple and efficient method for in vivo screening and evaluation of anti-tuberculosis drugs. Animal experiments were carried out in accordance with the provisions for animal ethics in the Regulations on Laboratory Animals of Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences.
