Genistein regulates miR-21 to promote the apoptosis in LPS-activated RAW264.7 cells
10.16438/j.0513-4870.2018-0744
- VernacularTitle:金雀异黄素调控miR-21表达促进LPS活化的RAW264.7细胞凋亡
- Author:
Li-ping XIANG
1
;
Li CONG
1
;
Yong ZHANG
1
;
Su-juan LIU
1
;
Xiao-lin XIE
1
;
Ping-juan BO
1
;
Xue-ping XIANG
1
;
Xiao-hua FU
1
Author Information
1. School of Medicine, Hunan Normal University, Changsha 410013, China
- Publication Type:Research Article
- Keywords:
genistein;
RAW264.7 cell;
lipopolysaccharide;
miR-21;
apoptosis
- From:
Acta Pharmaceutica Sinica
2019;54(2):281-287
- CountryChina
- Language:Chinese
-
Abstract:
The research is aimed to investigate the effect of genistein (GEN) on the apoptosis in lipopolysaccharide (LPS)-activated RAW264.7 cells and explore the pharmacological mechanism of GEN anti-atherosclerosis (AS). RAW264.7 cells were activated by LPS, the level of TNF-α and IL-6 mRNA were detected by qRT-PCR, the expression of COX-2 and iNOS were detected by Western blot. RAW264.7 cells were pretreated with GEN for 2 h, and then incubated with LPS for 24 h. After that, CCK8 kit was used for the cell viability, Annexin V-FITC/PI kit for the apoptosis of cell. qRT-PCR was used to detect the level of CHOP, caspase-3 and miR-21. Western blot was used to detect the expression of CHOP and caspase-3. Results showed that LPS (1 000 ng·mL-1) increased the expression of TNF-α, IL-6, COX-2 and iNOS in RAW264.7 cells compared with that in control group. GEN inhibited the cell activity and the level of miR-21, promoted the expression of CHOP and caspase-3 in LPS-activated RAW264.7 cells in a dose-dependent manner. miR-21 up inhibited the expression of CHOP and caspase-3 in LPS-activated RAW264.7 cells and this process was reversed by GEN treatment. miR-21 down promoted the expression of CHOP and caspase-3, which were further enhanced by GEN. These results indicate that GEN promotes the apoptosis of RAW264.7 cells activated by LPS through down regulating miR-21 and activating endoplasmic reticulum (ER) stress pathway.
