Feasibility exploration of discriminating red yeast rice for different applications by secondary metabolite fingerprints
10.16438/j.0513-4870.2018-0644
- VernacularTitle:用次生代谢物指纹图谱法区分不同用途红曲的可行性探索
- Author:
Ming-juan WANG
1
;
Shuai KANG
1
;
Xuan LIU
2
;
Xu WANG
2
;
Peng LIU
1
;
Zhong DAI
1
;
Shuang-cheng MA
1
Author Information
1. National Institutes for Food and Drug Control, Beijing 100050, China
2. College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
- Publication Type:Research Article
- Keywords:
red yeast rice;
quality control;
principal component analysis;
secondary metabolites;
ultra performance liquid chromatography
- From:
Acta Pharmaceutica Sinica
2019;54(2):354-359
- CountryChina
- Language:Chinese
-
Abstract:
Though red yeast rice (RYR) has been used as medicine for centuries, few study has been reported about its biological activities related to traditional medicinal application and marketed RYR showed poor consistency in quality. In this study, with comprehensive investigation of their production processes and field acquisition samples including those from genuine producing area, an ultra performance liquid chromatographic (UPLC) method was firstly established to discriminate RYR for different applications based on their secondary metabolites fingerprint. It was performed on a CAPCELL CORE AQ column (100 mm×4.6 mm, 2.7 μm), with PDA (range: 200-650 nm, extracted: 237 nm) and ELSD detection. The mobile phase used was water (A) and acetonitrile (B) both containing 0.1% formic acid at gradient elution (0-15 min, 50% B→85% B (linear); 15-16 min, 85 % B→50% B (linear) and maintained until 21 min), with a flow rate of 0.5 mL∙min-1. The method established was fully validated in agreement with guidelines of Chinese Pharmacopeia. Common metabolites were found in RYR for same application and the fingerprints of RYR for food coloring or brewing from various manufacturers had similarities above 0.90. Meanwhile, significant differences were observed among the fingerprints for various applications and discrimination could be achieved by principal component analysis (PCA). Lovastatin was absence in RYRs for food coloring or brewing, and the fingerprint of traditional medicinal RYR was similar to that of RYR for brewing. However, standardization was required for RYR containing lovastatin because of their significant differences from various manufacturers in fingerprints and lovastatin content. The results demonstrated the feasibility to discriminate RYR for different applications by the secondary metabolites fingerprint method established in this study, which provides a scientific basis to investigate the relationship between biological activities of medicinal RYR and their corresponding secondary metabolites, and further aid their quality standardization and improvement.
