Relative molecular weight determination of astragalus polysaccharides for injection
10.16438/j.0513-4870.2018-0612
- VernacularTitle:注射用黄芪多糖相对分子质量测定方法的比较及研究
- Author:
Ying WANG
1
;
Wei-yi XU
1
;
Li-xiao LI
2
;
Hong-yu JIN
1
;
Jian NI
3
;
Shuang-cheng MA
1
Author Information
1. National Institutes for Food and Drug Control, Beijing 100050, China
2. Shimadzu Co., LTD, Shanghai 201206, China
3. Beijing University of Chinese Medicine, Beijing 100029, China
- Publication Type:Research Article
- Keywords:
astragalus polysaccharides for injection;
relative molecular weight;
gel-chromatography;
multi-angle laser light scattering detection;
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
- From:
Acta Pharmaceutica Sinica
2019;54(2):348-353
- CountryChina
- Language:Chinese
-
Abstract:
To determine relative molecular weight of astragalus polysaccharides (APs), we used Shodex GS620 gel permeation chromatographic column and differential refraction detector (GPC-RI) with dextran as a reference. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and GPC combined with multi-angle laser light scattering detection (GPC-MALLS) were also used．GPC-RI measure showed four peaks of APs, with the Mw of 1 380 000, 231 000, 18 000, and 480, respectively. Three main peaks were found by GPC-RI-MALLS with the Mw as 1 148 000, 180 000, and 43 000, respectively. Strong signals in 155 000 and 18 000 were detected by MALDI-TOF-MS, which also indicated the sugar moieties of the APs as hexoses. From our study, we found that the GPC-RI method with universal correction is most suitable for Mw determination of the APs. Nevertheless, the three methods should be combined and contrasted with each other to obtain accurate information in research of polysaccharides from Chinese medicine.
