Expression analysis of Hsp70-related miRNA in Lonicera japonica Thunb. during different flowering stages
10.16438/j.0513-4870.2018-0389
- VernacularTitle:不同开花期金银花Hsp70基因相关miRNA的表达研究
- Author:
Feng-jie ZHU
1
;
Juan LIU
2
;
Yuan YUAN
2
;
Jun-hui ZHOU
2
;
Nian-jun YU
1
;
Lu-qi HUANG
2
Author Information
1. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
2. National Resource Center for Chinese Meteria Medica, China Academy of Chinese Medicine Sciences, Beijing 100700, China
- Publication Type:SPECIAL REPORTS
- Keywords:
Lonicera japonica;
miRNA;
Heat shock protein 70;
qRT-PCR
- From:
Acta Pharmaceutica Sinica
2018;53(8):1202-1208
- CountryChina
- Language:Chinese
-
Abstract:
Plant flowering regulation is an important mechanism to response to environmental stress. Heat shock protein 70 family is one of the main molecular chaperones to resist stress; miRNA can be used as a negative regulator to participate in post-transcriptional gene in flowering network. In this paper, we obtained an Hsp70 gene from Lonicera japonica transcriptome and combined with Lonicera japonica miRNA library to obtain a novel miRNA that may target Hsp70 gene through bioinformatics method. Bioinformatics and expression during different flowering stages of the obtained Hsp70 gene and miRNA were analyzed. Phylogenetic tree showed that the obtained Hsp70 gene was clustered with Hsp110 subfamily in Oryza sativa and Arabidopis thaliana. The prediction of miRNA secondary structure showed its stable structure and high reliability. The binding site map showed that there were two base mismatches between sequences of miRNA and Hsp70 gene. The expression analysis showed that the expression of Hsp70 and miRNA in different flowering stages had opposite trends, indicating that miRNA might regulate Hsp70 to participate in the flowering stages of Lonicera japonica. This study provided new ideas for Lonicera japonica flowering regulation and response to environmental stress mechanisms.
