Non-targeted metabolomics in septic mice infected with <i>Klebsiella pneumoniae</i>

VernacularTitle:肺炎克雷伯杆菌感染小鼠脓毒症模型的非靶向代谢组学研究
Author: Jia-xuan ZHANG ¹ ; Lang SUN ¹ ; Jing PANG ¹ ; Xin-xin HU ¹ ; Tong-ying NIE ¹ ; Xi LU ¹ ; Xiu-kun WANG ¹ ; Xin-yi YANG ¹ ; Xue-fu YOU ¹ ; Cong-ran LI ¹
Author Information

1. Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing 100050, China
Publication Type:ORIGINAL ARTICLES
Keywords: sepsis; metabolomics; UHPLC-QTOF-MS; biomarker
From: Acta Pharmaceutica Sinica 2018;53(7):1122-1130
CountryChina
Language:Chinese
Abstract: UHPLC-QTOF-MS was applied to non-targeted metabolomics study of mice infected with K. pneumoniae ATCC^® BAA 2146 to discover potential biomarkers and metabolic pathways that are associated with sepsis. Fifty-eight metabolites were identified by principal components analysis (PCA) and partial least-squares discriminant analysis (OPLS-DA), which was combined with variable projection importance (VIP) and nonparametric test. Eighteen of the 58 metabolites were further found to be involved in 8 metabolic pathways, including nicotinate and nicotinamide metabolism, pyrimidine metabolism, vitamin B6 metabolism, taurine and hypotaurine metabolism, arginine and proline metabolism, alanine, aspartate and glutamate metabolism, D-glutamine and D-glutamate metabolism and glycerophospholipid metabolism.