Impacts of particle shape on cellular uptake and tumor imaging application of ultra-pH sensitive micelles
10.16438/j.0513-4870.2018-0195
- VernacularTitle:pH超敏感纳米胶束的形状对其细胞摄取效率及肿瘤精确诊断的影响
- Author:
Zeng-hui WANG
1
;
Bin-long CHEN
1
;
Qing-qing YIN
1
;
Yue YAN
1
;
De-chun LIU
1
;
Qiang ZHANG
1
;
Yi-guang WANG
1
Author Information
1. School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
micelles;
ultra-pH sensitive;
fluorescence;
diagnosis
- From:
Acta Pharmaceutica Sinica
2018;53(5):797-805
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to investigate the impacts of particle shape of micelles on cell uptake and tumor imaging applications. We designed and synthesized an ultra-pH sensitive PEG-iPDPA diblock polymer, and prepared it into wormlike micelles and spherical micelles by thin-film dispersion method and modified solvent evaporation method, respectively. Firstly, the pH responsiveness of two kinds of micelles was investigated in vitro. Both forms of micelles responded to pH sensitively, and each of them could reach 100 times of ON/OFF fold after conjugated with BDP fluorescent probe. Moreover, the cellular uptake of two forms of micelles depended on the concentration and incubation time. However, the amount of cellular uptake of spherical micelles was much higher than that of the wormlike micelles, which proved that the shape of nanoparticles had a great influence on the cellular uptake. The results of in vivo imaging revealed that the spherical micelles had a better tumor accumulation as well as tumor imaging outcomes. Finally, the biosafety of micelles was tested by MTT assay and H&E staining, which indicated that none of the two kinds of micelles had obvious toxicity. Collectively, these results suggest that the spherical micelles could be a better carrier compared with wormlike micelles in terms of cellular uptake, tumor accumulation and tumor detection.
