Identification and analysis of the calcium signaling system genes responding to continuous cropping injury in Rehmannia glutinosa L.
10.16438/j.0513-4870.2017-0488
- VernacularTitle:地黄钙信号体系相关基因的鉴定及其在连作条件下响应特征分析
- Author:
Chu-yun YANG
1
;
Xin-yu LI
2
;
Ming-jie LI
1
;
Fa-jie FENG
1
;
Li GU
1
;
Bao ZHANG
1
;
Shu-qiang CHEN
1
;
Ai-guo CHEN
1
;
Zhong-yi ZHANG
1
Author Information
1. College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2. Agricultural College, Henan Agricultural University, Zhengzhou 450002, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Rehmannia glutinosa L;
continuous cropping injury;
autotoxicity;
calcium signaling system;
calcium ion concentration
- From:
Acta Pharmaceutica Sinica
2017;52(12):1951-1961
- CountryChina
- Language:Chinese
-
Abstract:
Calcium signaling plays a critical role in response to various abiotic and biotic stresses in plants. Preliminarily evidence showed that calcium signaling perceived and transduced the harmful signaling generated from continuous cropping stress in R. glutinosa. To investigate the roles of calcium signaling in continuous cropping injury formation, the key genes involved in calcium signaling transduction were identified in R. glutinosa transcriptome through bioinformatic methods. Furthermore, the calcium ion concentration in both normal and continuous cropping R. glutinosa root cells were measured by potassium pyroantimonate precipitation and calcium fluorescence method. As a result, a set of 84 calcium signaling-related genes, including 5 CaMs, 12 CBLs, 21 CDPKs, 21 CIPKs, 16 CMLs, and 9 CRKs were captured in R. glutinosa transcriptome. The analysis of expression profile in continuous cropping compared to normal growth R. glutinosa indicated that continuous cropping stress significantly increased the expression of calcium signaling-related genes in continuous cropping R. glutinosa. At the same time, the abundance levels of 12 calcium signaling-related genes quantified by qPCR further validated the high expression of calcium signaling-related genes presented in continous cropping R. glutinosa. In addition, the continuous cropping condition significantly promoted the accumulation of intracellular calcium ions in R. glutinosa based on two methods of potassium pyroantimonate precipitation and calcium fluorescence. This study verified the possible roles of calcium signaling in the formation of continuous cropping injury on molecular and cellular level, which lays a solid foundation for illuminating formation mechanism of continuous cropping injury on molecular level.
