Studies on site-directed mutagenesis of BmK AngM1 from scorpion venom and its anti-inflammatory activity
10.16438/j.0513-4870.2017-0222
- VernacularTitle:蝎毒活性肽BmK AngM1的定点突变及其抗炎活性研究
- Author:
Lan LIANG
1
;
Qing-hua WANG
1
;
Zong-feng HU
1
;
Ping ZHU
1
;
Qi HOU
1
;
Jin-ling YANG
1
Author Information
1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines and Key Laboratory of Biosynthesis of Natural Products of National Health and Family Planning Commission, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
scorpion toxin;
chitin;
site-directed mutagenesis;
anti-inflammatory activity
- From:
Acta Pharmaceutica Sinica
2017;52(6):1007-1011
- CountryChina
- Language:Chinese
-
Abstract:
Scorpion toxin BmK AngM1 has been reported to have a strong analgesic effect. However, its anti-inflammatory activity was unknown. In this study, the recombinant BmK AngM1 (rBmK AngM1) was expressed in Escherichia coli BL21 trxB (DE3). The purified rBmK AngM1 was obtained efficiently through the IMPACTTM-TWIN system. The anti-inflammatory activity of the recombinant protein was investigated. In order to improve the anti-inflammatory activity of rBmK AngM1, the potential active sites (Y5, Y42, R58) were substituted with different amino acids. The results showed that rBmK AngM1 and its mutants all have significant anti-inflammatory activity. The activities were significantly increased in the single mutant R58N and mutants Y5F/R58N, Y42F/R58N over the wild type protein. The data suggest that position 58 in BmK AngM1 plays a functional role in the anti-inflammatory activity. This study lays a foundation for the protein engineering design of BmK AngM1 to improve its pharmacological activity.
