Bioactive consistency evaluation of cardiotonic pill using marker genes
10.16438/j.0513-4870.2016-0115
- VernacularTitle:基于指标基因评价复方丹参滴丸生物活性一致性
- Author:
Wan-yang SUN
1
;
Ling TONG
2
;
Dong-xiang LI
2
;
Kai-shun BI
1
Author Information
1. Department of Pharmaceutical Analysis, Shenyang Pharmaceutical University, Shenyang 110016, China
2. State Key laboratory of Core Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Analysis Institute, Tasly Academy, Tianjin 300410, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
cardiotonic pill;
gene expression;
consistency;
bioactivity
- From:
Acta Pharmaceutica Sinica
2016;51(8):1290-
- CountryChina
- Language:Chinese
-
Abstract:
The study is aimed to develop a method in evaluation of the bioactive consistency of cardiotonic pill (CP). HepG2 cell line was employed as a biological detector. After treated with CP for 24 h, gene chip and qRT-PCR were used to select mRNAs that can represent the bioactivity of CP. Then similarity between different batches of CP were calculated based on expression levels of marker genes to evaluate the bioactive consistency of CP. Marker genes were selected according to the criteria as follows:① fold change <0.67 or >1.5; ② potential relevance to curative effects; ③ extensive involvement in the cellular functions and clustering analysis categories; ④ dose-dependent effect. A total of 10 genes were selected as bioactive markers of CP. Angular cosine was calculated to evaluate the similarity between two samples. The method was validated using intra-day precision and inter-day precision. Using angular cosine similarity, the intra-day and inter-day precision were 0.4% and 0.6%, respectively. The similarities of 6 batches of CDPs ranged from 0.992 to 0.999, and 1 batch of Compound Danshen Tablet was 0.534. The established method is specific and accurate, and provides comprehensive and objective evaluation of bioactive quality of CDPs. It can also benefit the bioactive consistency evaluation of other compounds in traditional Chinese medicines.
