Evaluation of cryptotanshinone inhibition of angiogenesis in human hepatic sinusoidal endothelial cells
10.16438/j.0513-4870.2016-0140
- VernacularTitle:基于肝窦内皮细胞功能评价隐丹参酮抑制血管新生的作用
- Author:
Kai HUANG
1
;
Zhi-min ZHAO
1
;
Hong-liang LIU
2
;
Xin SUN
2
;
Jing LÜ
2
;
Yan-yan TAO
2
;
Cheng-hai LIU
1
Author Information
1. Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai 201203, China
2. Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
cryptotanshinone;
liver sinusoidal endothelial cell;
tube formation;
zebrafish;
angiogenesis;
liver fibrosis
- From:
Acta Pharmaceutica Sinica
2016;51(8):1257-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of cryptotanshinone (an active ingredient of Salvia Miltiorrhiza) inhibition of angiogenesis, the toxicity of cryptotanshinone was assayed in human hepatic sinusoidal endothelial cells (HHSEC) by CCK8 method. Max dose without toxicity is 10 μmol·L-1. The proliferation of HHSEC were induced by the endothelial cell growth supplement (ECGS), with 2.5 μmol·L-1 sorafenib as the positive control. Cell proliferation was analyzed by EdU assay. Cell viability was analyzed by CCK8 method. The expression of vWF was analyzed by immunofluorescence method. Fluorescence probe method was used to detect the intracellular nitric oxide (NO) levels. Tube formation of HHSEC and transgenic zebrafish were also observed to evaluate the effects of cryptotanshinone against angiogenesis. Compared with normal control, there is a proliferation of HHSEC induced by ECGS. The expression of vWF and the NO levels increased significantly. Cryptotanshinone inhibited the proliferation, down regulated the expression of vWF and the NO levels. Further, cryptotanshinone inhibited the tube formation of HHSEC and reduced the number of fu nctional vessels in transgenic zebrafish. The results suggest that cryptotanshinone could inhibit angiogenesis by regulating the HHSEC cell function.
