Evaluation of chemical consistency of triterpene acids in ethanol extracts of Poria and acetic ether extracts thereof by UPLC-QTOF-MS/MS with full scan and mimic MRM mode
10.16438/j.0513-4870.2018-0670
- VernacularTitle:整合UPLC-QTOF-MS/MS全扫描和模拟MRM方法综合评价茯苓乙醇提取物与后续乙酸乙酯萃取物三萜酸类组分化学一致性
- Author:
Ye-ting ZOU
1
;
Jin-di XU
2
;
Fang LONG
3
;
Ye-qing ZHANG
4
;
Song-lin LI
1
Author Information
1. Department of Pharmaceutical Analysis, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine to Nanjing University of Chinese Medicine, Nanjing 210028, China; Department of Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of Chinese Academy of Traditional Chinese Medicine, Nanjing 210028, China
2. Department of Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of Chinese Academy of Traditional Chinese Medicine, Nanjing 210028, China
3. Department of Pharmaceutical Analysis, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine to Nanjing University of Chinese Medicine, Nanjing 210028, China; Department of Respiratory Medicine, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine to Nanjing University of Chinese Medicine, Nanjing 210028, China
4. Department of Respiratory Medicine, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine to Nanjing University of Chinese Medicine, Nanjing 210028, China
- Publication Type:Research Article
- Keywords:
Poria;
triterpene acid;
liquid-liquid extraction;
chemical consistency;
UPLC-QTOF-MS/MS
- From:
Acta Pharmaceutica Sinica
2019;54(1):130-137
- CountryChina
- Language:Chinese
-
Abstract:
An ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) method was developed to evaluate the chemical consistency of triterpene acids in ethanol extracts of Poria and acetic ether extracts thereof. First, high resolution mass spectrometry data were obtained with Full scan mode, by comparing with MS data from the reference compounds and literatures, a total of 23 components were unequivocally or tentatively identified in ethanol extracts and acetic ether extracts thereof. Then, a mimic multiple reaction monitoring (mMRM) mode was established using UPLC-QTOF-MS/MS to quantify the triterpene acids in ethanol extracts and acetic ether extracts thereof. Eleven components were absolutely quantified with reference compounds, while 12 components without reference compounds were relatively quantified with peak areas, the transfer and enrichment rate of triterpene acids during liquid-liquid extraction were calculated. It was found all of the 23 triterpene acids identified in Poria ethanol extracts could be transferred into acetic ether extracts with high transfer and enrichment rate. The present study provides not only scientific evidence for further extraction of triterpene acids in Poria by acetic ether, but also an approach for comprehensive evaluation of the chemical consistency of herbal medicine extracts before and after the liquid-liquid extraction.
