Effect of loureirin A against Candida albicans biofilms.
10.1016/S1875-5364(19)30064-0
- Author:
Mei-Yu LIN
1
;
Zhong-Lan YUAN
1
;
Dan-Dan HU
1
;
Gan-Hai HU
2
;
Ri-Li ZHANG
1
;
Hua ZHONG
1
;
Lan YAN
1
;
Yuan-Ying JIANG
1
;
Juan SU
3
,
4
;
Yan WANG
4
,
5
Author Information
1. School of Pharmacy, Naval Military Medical University, Shanghai 200433, China.
2. School of Pharmacy, Naval Military Medical University, Shanghai 200433, China; Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350000, China.
3. School of Pharmacy, Naval Military Medical University, Shanghai 200433, China. Electronic address: juansu_2008@
4. com.
5. School of Pharmacy, Naval Military Medical University, Shanghai 200433, China. Electronic address: wangyansmmu@
- Publication Type:Journal Article
- Keywords:
Anti-biofilm;
Candida albicans;
Loureirin A;
Morphological transition
- From:
Chinese Journal of Natural Medicines (English Ed.)
2019;17(8):616-623
- CountryChina
- Language:English
-
Abstract:
Loureirin A is a major active component of Draconis sanguis, a traditional Chinese medicine. This work aimed to investigate the activity of loureirin A against Candida albicans biofilms. 2, 3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT)reduction assay and scanning electron microscopy were used to investigate the anti-biofilm effect. Minimal inhibitory concentration testing and time-kill curve assay were used to evaluate fungicidal activity. Cell surface hydrophobicity (CSH) assay and hyphal formation experiment were respectively carried out to investigate adhesion and morphological transition, two virulence traits of C. albicans. Real-time RT-PCR was used to investigate gene expression. Galleria mellonella-C. albicans and Caenorhabditis elegans-C. albicans infection models were used to evaluate the in-vivo antifungal effect. Human umbilical vein endothelial cells and C. elegans nematodes were used to evaluate the toxicity ofloureirin A. Our data indicated that loureirin A had a significant effect on inhibiting C. albicans biofilms, decreasing CSH, and suppressing hyphal formation. Consistently, loureirin A down-regulated the expression of some adhesion-related genes and hypha/biofilm-related genes. Moreover, loureirin A prolonged the survival of Galleria mellonella and Caenorhabditis elegans in C. albicans infection models and exhibited low toxicity. Collectively, loureirin A inhibits fungal biofilms, and this effect may be associated with the suppression of pathogenic traits, adhesion and hyphal formation.
