Hematopoietic effect of small molecular fraction of Polygoni multiflori Radix Praeparata in cyclophosphamide-induced anemia mice.
10.1016/S1875-5364(19)30075-5
- Author:
Li-Li ZHU
1
;
Luo-Di FAN
2
;
Ming-Hua HU
2
;
Fang-Li MA
2
;
Jin QI
3
,
4
Author Information
1. Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing 210009, China.
2. Infinitus Chinese Herbal Immunity Research Centre, Infinitus (China) Company Limited., Guangzhou 510663, China.
3. Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing 210009, China. Electronic address: qijin2006@
4. com.
- Publication Type:Journal Article
- Keywords:
Cyclophosphamide;
Hemopoiesis;
Polygoni multiflori Radix Praeparata;
Small molecular fraction
- From:
Chinese Journal of Natural Medicines (English Ed.)
2019;17(7):535-544
- CountryChina
- Language:English
-
Abstract:
The aim of this study is to investigate the protective effects of a small molecular fraction (SMF) of Polygoni multiflori Radix Praeparata (PMRP) in a cyclophosphamide (CTX) induced anemia mouse model. Small molecular fraction of PMRP was prepared and identified by high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS). In pharmacology, we examined the peripheral hemogram and thymus and spleen index. The content of granulocyte-macrophage colony-stimulating factor (GM-CSF) in serum was mensurated by enzyme-linked immunosorbent assay (ELISA); The level of superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (T-AOC), and malondialdehyde (MDA) in serum and spleen tissue homogenate were detected, and glutathione peroxidase (GSH-PX) was assayed in spleen. The results show that SMF can significantly accelerate the recovery of peripheral hemogram, increase the activity of antioxidant enzymes and GM-CSF in serum and spleen. SMF also increases the number of spleen cells, improves bone marrow pathology. In conclusion, the SMF of PMRP promoted the recovery of hematopoietic function in a CTX-induced anemia mouse, which can support SMF to be used as an adjunct to chemotherapy to counteract its side effects.
