Systems-Based Interactome Analysis for Hematopoiesis Effect of Angelicae sinensis Radix: Regulated Network of Cell Proliferation towards Hemopoiesis.
10.1007/s11655-018-3003-5
- Author:
Guang ZHENG
1
,
2
;
He ZHANG
3
;
Yun YANG
3
;
Ying-Li SUN
4
;
Yan-Jing ZHANG
3
;
Ju-Ping CHEN
3
;
Ting HAO
3
;
Cheng LU
5
;
Hong-Tao GUO
6
;
Ge ZHANG
7
;
Dan-Ping FAN
5
;
Xiao-Juan HE
5
;
Ai-Ping LU
7
Author Information
1. Information Science and Engineering School, Lanzhou University, Lanzhou, 730000, China. forzhengguang@
2. com.
3. Information Science and Engineering School, Lanzhou University, Lanzhou, 730000, China.
4. School of Biology, Lanzhou University, Lanzhou, 730000, China.
5. Institute of Basic Research in Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
6. Department of Rheumatism, the First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, 450000, China.
7. School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, China.
- Publication Type:Journal Article
- Keywords:
Angelicae sinensis Radix;
cell proliferation;
cross-talking agents;
hemopoiesis;
interactome;
regulation
- From:
Chinese journal of integrative medicine
2019;25(12):939-947
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To explore the molecular-level mechanism on the hematopoiesis effect of Angelicae sinensis Radix (ASR) with systems-based interactome analysis.
METHODS:This systems-based interactome analysis was designed to enforce the workflow of "ASR (herb)→compound→target protein→internal protein actions→ending regulated protein for hematopoiesis". This workflow was deployed with restrictions on regulated proteins expresses in bone marrow and anemia disease and futher validated with experiments.
RESULTS:The hematopoiesis mechanism of ASR might be accomplished through regulating pathways of cell proliferation towards hemopoiesis with cross-talking agents of spleen tyrosine kinase (SYK), Janus kinase 2 (JAK2), and interleukin-2-inducible T-cell kinase (ITK). The hematopoietic function of ASR was also validated by colony-forming assay performed on mice bone marrow cells. As a result, SYK, JAK2 and ITK were activated.
CONCLUSION:This study provides a new approach to systematically study and predict the therapeutic mechanism for ASR based on interactome analysis towards biological process with experimental validations.
