Effects of CeA lesions on the initiation and expression of sodium appetite in sodium-deficient rats.
10.12047/j.cjap.5749.2019.004
- Author:
Zhi Xin ZHAO
1
;
Ying Ying LIAO
1
;
Yuan Yuan FAN
2
;
En She JIANG
1
Author Information
1. Institute of Nursing and Health, Henan University, Kaifeng
2. College of Life Science, Henan University, Kaifeng 475004, China.
- Publication Type:Journal Article
- MeSH:
Amygdala;
pathology;
Animals;
Appetite;
Diet, Sodium-Restricted;
Neurons;
Rats;
Rats, Sprague-Dawley;
Sodium;
Sodium, Dietary;
pharmacology
- From:
Chinese Journal of Applied Physiology
2019;35(1):13-18
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of central nucleus of amygdala (CeA) lesion on the initiation and expression of sodium appetite in sodium-deficient rats.
METHODS:Three groups of SD rats (n=6 in each group) were treated with bilateral CeA lesion, sham lesion or no lesion. After the recovery, the rats were fed with low-sodium diets for 14 days to establish a sodium-deficient rat model. The double-bottle selection in single cage test was used to observe the intake of 0.3 mol/L NaCl and DW in 5 timepoint with 24 hours in sodium-deficient rats. Immunofluorescence staining of aldosterone-sensitive neurons in the nucleus tractus solitarii (NTS)was used to investigate the effect of CeA lesion or not on the activity of aldosterone-sensitive neurons in rats with or without sodium deficiency.
RESULTS:After fed with low-sodium diet for14 days, the volume and preference rate of 0.3 mol/L NaCl intake of the rats within 24 h were significantly increased compared with those before low-sodium diet (P＜0.01). The intake volume and the preference rate of 0.3 mol/L NaCl in CeA lesion rats were significantly decreased than those in CeA sham lesion rats and normal rats in the sodium-deficient condition (P＜0.01). The CeA lesion had no effects on the activity of aldosterone-sensitive neurons in NTS in rats with low-sodium diet.
CONCLUSION:Low-sodium diet induces an increase in the expression of sodium appetite in rats. CeA lesions inhibit the behavioral expression of sodium appetite in sodium-deficient rats but have no effects on the initiation of sodium appetite in rats with sodium-deficient rats.
