Identification of chemical constituents in Sinopodophylli Fructus by HPLC-DAD-ESI-IT-TOF-MSn.
10.19540/j.cnki.cjcmm.20171027.003
- Author:
Ai-Hua WANG
1
;
Li-Man MA
1
;
Shan-Shan FAN
1
;
Guang-Xue LIU
1
;
Feng XU
1
;
Ming-Ying SHANG
1
;
Shao-Qing CAI
1
Author Information
1. Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
- Publication Type:Journal Article
- Keywords:
HPLC-DAD-ESI-IT-TOF-MSn;
Sinopodophylli Fructus;
flavonoids;
lignan
- MeSH:
Berberidaceae;
chemistry;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
chemistry;
Flavonoids;
analysis;
Fruit;
chemistry;
Lignans;
analysis;
Phytochemicals;
analysis;
Spectrometry, Mass, Electrospray Ionization;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2018;43(1):123-133
- CountryChina
- Language:Chinese
-
Abstract:
This experiment was performed to analyze and identify the chemical constituents of Sinopodophylli Fructus by HPLC-DAD-ESI-IT-TOF-MSn. The analysis was performed on an Agilent Zorbax SB-C₁₈ (4.6 mm×250 mm, 5 μm) column.The mobile phase consisted of 0.1% formic acid was used for gradient at a flow rate of 1.0 mL·min⁻¹. Electrospray ionization ion trap time-of-flight multistage mass spectrometry was applied for qualitative analysis under positive and negative ion modes. The results indicated that 54 compounds consisted of 18 lignans and 36 flavonoids from Xiaoyelian had been detected by their HRMS data, the information of literature and reference substance. Among them, 27 compounds were reported in Sinopodophylli Fructus for the first time. In conclusion, an HPLC-DAD-ESI-IT-TOF-MSn method was established to qualitative analysis of Xiaoyelian in this study, which will provide the evidence for evaluating the quality of Xiaoyelian herbs, clarifying the mechanism, and guiding the development of pharmacological active ingredients.
