Design of recombinase and terminator-based genetic switches for cell state control.

Author: Songyuan ZHANG ¹ ; Jianhui QIU ¹ ; Xuan WANG ² ; Yiming DONG ¹ ; Yulong LI ¹ ; Yihao ZHANG ¹ ; Qi OUYANG ¹
Author Information

1. Center for Quantitative Biology and Center for Life Sciences, Peking University, Beijing 100871, China.
2. Center for Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China.
Publication Type:Journal Article
Keywords: genetic switch; site-specific recombinase; state transition; terminator
MeSH: Recombinases; metabolism
From: Chinese Journal of Biotechnology 2018;34(12):1874-1885
CountryChina
Language:Chinese
Abstract: Various genetic switches have been developed to let engineered cells perform designed functions. However, a sustained input is often needed to maintain the on/off state, which is energy-consuming and sensitive to perturbation. Therefore, we developed a set of transcriptional switches for cell states control that were constructed by the inversion effect of site-specific recombinases on terminators. Such a switch could respond to a pulse signal and maintain the new state by itself until the next input. With a bottom-up design principle, we first characterized the terminators and recombinases. Then the mutual interference was studied to select compatible pairs, which were used to achieve one-time and two-time state transitions. Finally, we constructed a biological seven-segment display as a demonstration to prove such switch's immense potential for application.