Effects of Different Inflammatory Factors on Hepatocyte Kinase Receptors and Ligands in Human Periodontal Ligament Fibroblasts.
10.3881/j.issn.1000-503X.10599
- Author:
Xiao Nan XU
1
;
Meng Lin WANG
1
;
Ding ZHANG
1
Author Information
1. Department of Stomatology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Ephrins;
metabolism;
Fibroblasts;
Humans;
Interleukin-1beta;
pharmacology;
Ligands;
Periodontal Ligament;
cytology;
Receptors, Eph Family;
metabolism;
Tumor Necrosis Factor-alpha;
pharmacology
- From:
Acta Academiae Medicinae Sinicae
2019;41(3):300-306
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different inflammatory factors on hepatocyte kinase receptor(Eph)and ligand(ephrin)in human periodontal ligament fibroblasts(hPDLFs).Methods hPDLFs were stimulated with either 10 ng/ml tumor necrosis factor-α(TNF-α)or 10 ng/ml interleukin(IL)-1β,and then the expressions of Eph and ephrin at both mRNA and protein levels were determined at 0,1,2,6,12,and 24 hours.Results The levels of Eph receptors and ephrin ligand changed in a time-dependent manner in human periodontal ligament fibroblasts after treatment with TNF-α or IL-1β. The expression of ephrinA2 significantly increased in both groups within 24 hours(all <0.05). In the TNF-α group,the mRNA expression of ephrinA2 significantly increased at 1 h and was significant higher that in the IL-1β group at 24 h(<0.05). EphB4 showed a time-dependent decline after a short period of high expression.Conclusions Both TNF-α and IL-1β can cause changes in the expressions of Eph receptors and ephrin ligands in hPDLFs. The changes induced by both are consistent,although the effect of TNF-α is more pronounced.
