Identification of A Case of DEL Blood Group with DAT Positive Phenotype and Pedigree Analysis.
10.19746/j.cnki.issn.1009-2137.2019.05.045
- Author:
Kai-Hua XIANG
1
;
Hui-Ying XU
2
;
Chun-Hua LIU
1
;
Rui WANG
1
;
Shi-Fang YU
1
;
Pan YU
1
;
Lu-Fei XU
1
;
Zhi-Hua TAO
3
;
Lu-Fei YU
4
Author Information
1. Department of Blood Transfusion,The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou,310009, Zhejiang Province, China.
2. Department of Laboratorial Examnation, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou,310009, Zhejiang Province, China.
3. Department of Laboratorial Examnation, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou,310009, Zhejiang Province, China E-mail：zrtzh@zju.edu.cn.
4. Department of Blood Transfusion,The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou,310009, Zhejiang Province, China E-mail: 2514117@zju.edu.cn.
- Publication Type:Case Reports
- MeSH:
Alleles;
Exons;
Genotype;
Humans;
Pedigree;
Phenotype;
Rh-Hr Blood-Group System
- From:
Journal of Experimental Hematology
2019;27(5):1649-1653
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To identify the blood group of a patient with DEL phenotype combined with positive direct anti-human globulin test and to analyze the pedigree.
METHODS:Routine serological reagents were used for serological analysis of RhD blood group in the pedigree members. Exons and flanking sequences of RHD gene were amplified, sequenced and analyzed for heterozygosity. The familial genetic state of DEL phenotype was further analyze in the family members.
RESULTS:The DAT was strongly positive in the proband. The 1227G>A allele (RHD*DEL1) was present in the exon 9 of RHD gene, and the mother was the carrier of RHD*DEL1. The proband was identified as RHD+/RHD-, suggesting the CDe/Cde haplotype.
CONCLUSION:The proband is DEL phenotype (RHD*DEL1).
