Effects of shading on key enzyme genesexpression and accumulation of saponins in Panax japonicus var. major.
10.19540/j.cnki.cjcmm.20180528.007
- Author:
Wen-Jing HUANG
1
;
Xiao-Chun SUN
1
;
Bo LI
1
;
Xin-Bo SHI
1
;
Zheng-Gang YUE
1
;
Xing-Hang CAI
1
;
Jie YANG
1
;
Zhi-Shu TANG
1
Author Information
1. Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xianyang 712083, China.
- Publication Type:Journal Article
- Keywords:
Panax japonicus var. major;
gene expression;
shading;
triterpenoid saponins
- MeSH:
Gene Expression Regulation, Plant;
Light;
Panax;
enzymology;
genetics;
radiation effects;
Plant Leaves;
enzymology;
genetics;
Rhizome;
enzymology;
genetics;
Saponins;
analysis
- From:
China Journal of Chinese Materia Medica
2018;43(19):3855-3861
- CountryChina
- Language:Chinese
-
Abstract:
To explore the effects of shading and the expression of key enzyme genes on the synthesis and accumulation of Panax japonicus var. major saponins, different shading treatments (0%, 30%,50%) of potted P. japonicus var. major were used as test materials, the expression of three key enzyme genes(CAS,DS,-AS) of leaves and rhizomes in different growth periods of P. japonicus var. major was determined by real-time quantitative PCR, the content of total saponins was determined by ultraviolet spectrophotometry. The results indicated that, in flowering stage, CAS,DS,-AS were highly expressed in the aerial parts of P. japonicus var. major, 30% shading treatment significantly inhibited the expression of CAS in leaves and promoted the expression of DS and -AS in stems, leaves and flowers, it was speculated that the main part of saponin synthesis was leaf in this stage. Both the expression levels of DS and -AS and changes in the content of total saponins in leaves showed a tendency of low-high-low throughout the growth cycle, correlation coefficient analysis showed that there was a positive correlation between them. Compared with control, the expression levels of DS and -AS and the content of total saponins were greatly enhanced under shading treatment, 30% shading treatment significantly promoted the accumulation of total saponins. Therefore, it is suggested that 30% shading treatment should be applied to the artificial cultivation of P. japonicus var. major, which is beneficial to the accumulation and quality improvement of saponins.
