Research of Feiliuping Gao and its combination with different types of drugs intervention on expression of PI3K/AKT/NF-κB in lung metastatic microenvironment.
10.19540/j.cnki.cjcmm.20180806.001
- Author:
Rui LIU
1
;
Hong-Gang ZHENG
1
;
Wei-Dong LI
1
;
Bao-Jin HUA
1
;
Bo YAO
2
;
Xin QI
1
;
Ying-Xia PEI
1
;
Yun ZHANG
1
Author Information
1. Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
2. Heilongjiang University of Chinese Medicine, Harbin 150040, China.
- Publication Type:Journal Article
- Keywords:
Feiliuping Gao;
PI3K;
celecoxib;
metastasis microenvironment;
traditional Chinese medicine
- MeSH:
Animals;
Carcinoma, Lewis Lung;
pathology;
Drugs, Chinese Herbal;
pharmacology;
Lung;
Mice;
NF-kappa B;
metabolism;
Neoplasm Metastasis;
Phosphatidylinositol 3-Kinases;
metabolism;
Proto-Oncogene Proteins c-akt;
metabolism;
Signal Transduction
- From:
China Journal of Chinese Materia Medica
2018;43(19):3913-3918
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this paper was to observe the effect of Feiliuping Gao and its combination with different types of drugs intervention on the expression of PI3K/AKT/NF-κB in lung metastatic microenvironment, and to reveal the advantage of Chinese medicine intervention time on the key molecule in lung metastatic microenvironment. The mouse model of Lewis lung carcinoma was established, and lung tissues were collected at 14 days, 21 days and 28 days after the intervention of Feiliuping Gao, and the expressions of PI3K, AKT and NF-κB were detected by immunohistochemistry and Western blot. At 14 days, there was no significant difference in PI3K expression between each group and the control group. The expression of AKT protein was significantly inhibited in the celecoxib (CLB) group, the Feiliuping Gao (FLP) combination with cyclophosphamide (FLP+CTX) group, and the Feiliuping Gao combination with celecoxib (FLP+CLB) group (<0.05). The inhibition of AKT protein expression in FLP+CLB group was superior. The FLP+CLB group can inhibit the expression of NF-κB protein (<0.05). At 21 days, compared with the control group, the expression of PI3K was inhibited in FLP group and the FLP+CTX group (<0.05), while the expression of PI3K was best inhibited in the FLP+CLB group (<0.001). Only the FLP+CLB group could significantly inhibit the expression of AKT protein (<0.01). The FLP+CTX group had the best effect in inhibiting the expression of NF-κB protein (<0.001). At 28 days, compared with the control group, the expression of PI3K and AKT was inhibited in the FLP+CLB group (<0.001). Feiliuping ointment combination with celecoxib has an advantage in regulating the expression of PI3K/AKT/NF-κB molecules in lung metastatic microenvironment.
