Effect of Huangdi Anxiao Capsules on zebrafish vascular lesions induced by high glucose and high fat.
10.19540/j.cnki.cjcmm.20180606.002
- Author:
Jia-Rong GAO
1
;
Ming-Fei GUO
1
;
Zhao-Hui FANG
1
;
Hui JIANG
1
;
Yi-Qiao XU
2
;
Le-Tian SHAN
3
;
Liang-Bing WEI
1
;
Li SHAN
1
Author Information
1. State Administration of Chinese Medicine Preparation Three Laboratories, First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031, China.
2. Hangzhou Hunter Biotechnology Inc, Hangzhou 310051, China.
3. Zhejiang Chinese Medical University, Hangzhou 310053, China.
- Publication Type:Journal Article
- Keywords:
Huangdi Anxiao Capsules;
diabetes mellitus;
high-sugar and high-fat;
vascular disease;
zebrafish
- MeSH:
Animals;
Capsules;
Diet, High-Fat;
adverse effects;
Drugs, Chinese Herbal;
pharmacology;
Glucose;
adverse effects;
Peptidyl-Dipeptidase A;
metabolism;
Random Allocation;
Vascular Diseases;
drug therapy;
pathology;
Vascular Endothelial Growth Factor A;
metabolism;
Zebrafish;
Zebrafish Proteins;
metabolism
- From:
China Journal of Chinese Materia Medica
2018;43(21):4317-4322
- CountryChina
- Language:Chinese
-
Abstract:
Zebrafish of different strains with 5 dpf (5 days post-fertilization) were selected and fed with 0.2% high-fat diet for 8 h and 3% glucose solution for 16 halternatively during the day and night for 4 consecutive days. The zebrafish model was established and randomly divided into model group, Huangdi Anxiao Capsules (260 mg·L⁻¹) group and pioglitazone (32 mg·L⁻¹) group. The drug treatment groups were given the water-soluble drugs, with a volume of 25 mL, and incubated in a 28 °C incubator for 4 days. To detect the exposure to the corresponding drugs, the normal control group was set up. Thirty zebrafish were included in each group. The effect of Huangdi Anxiao Capsules on vascular wall thickness, fluorescence intensity of islet beta cells, fluorescence intensity of macrophages, and blood flow velocity of zebrafish were detected. The expressions of vascular endothelial growth factor (vegfaa) and angiotensin converting enzyme (ACE) were detected by RT-PCR. The results showed that compared with the model group, Huangdi Anxiao Capsules can significantly reduce the thickness of the blood vessel wall, increase the fluorescence intensity of islet β cells and macrophages, increase the blood flow velocity in vivo, and decrease the ACE and vegfaa expressions in zebrafish. It is suggested that Huangdi Anxiao Capsules may alleviate zebrafish vascular lesions by regulating the expressions of ACE and vegfaa.
