Identification and attribution of chemical compounds of Pudilan Antiphlogistic Oral Liquid.
10.19540/j.cnki.cjcmm.20181221.009
- Author:
Li-Juan ZHAO
1
;
Wen-Ya GAO
1
;
Xin-Ru GU
1
;
Hong-Jie WANG
1
;
Hai-Yu ZHAO
1
;
Bao-Lin BIAN
1
Author Information
1. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
- Publication Type:Journal Article
- Keywords:
Pudilan Antiphlogistic Oral Liquid;
UPLC-ESI-Orbitrap-MS/MS;
alkaloids;
compounds identification;
flavonoids;
organic acids
- MeSH:
Anti-Inflammatory Agents;
analysis;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
chemistry;
Phytochemicals;
analysis;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2019;44(8):1573-1587
- CountryChina
- Language:Chinese
-
Abstract:
UPLC-ESI-Orbitrap-MS/MS was used to analyze,identify and attribute the chemical constituents in Pudilan Antiphlogistic Oral Liquid. The analysis was performed on an Agilent Eclipse XDB-C18(4.6 mm × 150 mm,3.5 μm) with a gradient mobile phase of methanol-0.1% formic solution system at the flow rate of 0.5 m L·min-1. The sample volume was 2 μL. The column temperature was30 ℃. The high-resolution orbitrap mass spectrometry was used as detector,with electrospray ion source in both positive and negative models,and the MS scanning ranged between m/z 50 and 2 000. Based on the analysis of mass spectrometry and literature reports,79 compounds were confirmed,including 30 alkaloids,28 organic acids,18 flavonoids and 3 coumarins. Finally,39 compounds,such as rutin,esculetin,gallic acid,caffeic acid,cichoric acid,were identified from Taraxacum mongolicum; 11 compounds,such as baicalin,baicalein,apigenin,chrysin,oroxylin A,were identified from Scutellaria baicalensis; 13 compounds,such as arginine,proline,hypoxanthine,epigoitrin,indirubin,were identified from Isatis indigotica; and 18 compounds,such as dehydrocheilanthifoline,oxysanguinarine,corynoline,protopine,spallidamine,were identified from Corydalis bungeana. After the analysis of chemical model and attribution,the contents of some compounds were high in Pudilan Antiphlogistic Oral Liquid,such as baicalin,wogonoside,baicalein,wogonin,apigenin,chrysin,skullcapflavonⅡ,oroxylin A,cichoric acid,chlorogenic acid,caffeic acid,esculetin,dehydrocheilanthifoline,dihydrosanguinarine,protopine,corynoline and indirubin. The established method is simple,accurate,rapid,sensitive and reproducible,and thus suitable for the qualitative identification and quantitative determination of Pudilan Antiphlogistic Oral Liquid,which lays a foundation for the systematic quality control and the establishment of whole-course traceability system of active ingredients.
