Garlicin Post-Conditioning Suppresses Adhesion Molecules in a Porcine Model of Myocardial Ischemia-Reperfusion Injury.
10.1007/s11655-017-2757-5
- Author:
Peng YANG
1
;
Jia-Hui LI
1
;
Ai-Li LI
1
;
Jing LI
1
;
Yong WANG
1
;
Shi-Yan REN
1
;
Xian-Lun LI
2
,
3
Author Information
1. Department of Cardiology, China-Japan Friendship Hospital, Beijing, 100029, China.
2. Department of Cardiology, China-Japan Friendship Hospital, Beijing, 100029, China. leexianlun@
3. com.
- Publication Type:Journal Article
- Keywords:
CD29;
CD31;
garlicin;
myocardial infarction;
reperfusion injury
- MeSH:
Allyl Compounds;
pharmacology;
Animals;
Disease Models, Animal;
Disulfides;
pharmacology;
Integrin beta1;
analysis;
genetics;
physiology;
Ischemic Postconditioning;
Male;
Myocardial Reperfusion Injury;
prevention & control;
Platelet Endothelial Cell Adhesion Molecule-1;
analysis;
antagonists & inhibitors;
genetics;
RNA, Messenger;
analysis;
Swine
- From:
Chinese journal of integrative medicine
2019;25(1):31-36
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To evaluate whether garlicin post-conditioning can attenuate myocardial ischemiareperfusion injury in a catheter-based porcine model of acute myocardial infarction (AMI) by affecting adhesion molecules integrin β1/CD29 and platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31).
METHODS:Twenty-two swine were devided into 3 groups: 6 in a sham-operation group, and 8 each in the model and garlicin groups. AMI porcine model was established in the model and garlicin groups. The distal parts of the left anterior descending coronary artery in the animals of the model and garlicin groups were occluded by dilated balloon for 2 h, followed by reperfusion for 3 h. Garlicin (1.88 mg/kg) was injected over a period of 1 h, beginning just before reperfusion, in the garlicin group. Real-time polymerase chain reaction, immunohistochemistry and Western blot were carried out to detect mRNA and protein expressions of CD29 and CD31 3 h after reperfusion.
RESULTS:Hematoxylin-eosin staining showed a better myocardial structure in the garlicin group after reperfusion. Compared to the model group, garlicin inhibited both the mRNA and protein expression of CD29 and CD31 in reperfusion area and no-reflflow area (P<0.05 respectively).
CONCLUSIONS:Garlicin post-conditioning induced cardio-protection against myocardial ischemia-reperfusion injury in this catheter-based porcine model of AMI. The cardio-protective effect of garlicin is possibly owing to suppression of production of CD29 and CD31, by inhibition of the mRNA expression of CD29 and CD31.
