Angiotensin Ⅱ induces apoptosis of HEK293T cells by up-regulating Cyr61 expression.
10.12122/j.issn.1673-4254.2019.07.10
- Author:
Junjie WANG
1
;
Yan JIANG
2
;
Senouthai SOULIXAY
1
;
Dongdong FU
1
;
Yanwu YOU
1
Author Information
1. Department of Nephrology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China.
2. Science Lab Center, Youjiang Medical University for Nationalities, Baise 533000, China.
- Publication Type:Journal Article
- Keywords:
CRISPR/Cas9;
Cyr61;
HEK293T cells;
angiotensin Ⅱ;
apoptosis
- MeSH:
Angiotensin II;
Apoptosis;
Cysteine-Rich Protein 61;
HEK293 Cells;
Humans;
Up-Regulation
- From:
Journal of Southern Medical University
2019;39(7):810-815
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the role of Cyr61 in angiotensin Ⅱ (AngⅡ)-induced functional changes in HEK293 cells and explore the mechanism.
METHODS:Cyr61 knockdown in cultured HEK293T cells was achieved by transfection of the cells with CRISPR/Cas9 KO plasmid. The changes in apoptosis and expression levels of Cyr61 and Bcl-2 in the cells with or without Cyr61 knockdown in response to treatment with 10 mol/L AngⅡ for 48 h were analyzed using flow cytometry, qRT-PCR and Western blotting.
RESULTS:The cells with Cyr61 knockdown showed significantly decreased expression of Cyr61 protein as compared with the control cells ( < 0.05). AngⅡ treatment for 48 h significantly increased the expression of Cyr61 and lowers the expression of Bcl-2 at both the protein and mRNA levels in HEK293T cells. In HEK293T cells with Cyr61 knockdown, AngⅡ treatment resulted in significantly increased expression of Bcl-2 in HEK293T cells as compared with that of the control group ( < 0.05). AngⅡ treatment caused significantly increased apoptotic rate in HEK293T cells as compared with the cells with Cyr61 knockdown [(26.94 ± 3.73)% (3.87 ± 0.83)%, < 0.05), and the apoptosis rate was significantly lowered to (15.76 ± 1.31)% in HEK293T cells with Cyr61 knockdown following AngⅡ treatment ( < 0.05).
CONCLUSIONS:The up-regulation of Cyr61 expression is related with AngⅡ-induced injury in HEK293T cells, and down-regulating Cyr61 expression can effectively protect HEK293T cells against AngⅡ-induced injury.
