Relationship between Maternal PBMC HBV cccDNA and HBV Serological Markers and its Effect on HBV Intrauterine Transmission.
- Author:
Dan Dan WANG
1
;
Lin Zhu YI
1
;
Li Na WU
1
;
Zhi Qing YANG
1
;
Hai Yun HAO
1
;
Xiao Hong SHI
1
;
Bo WANG
2
;
Shu Ying FENG
2
;
Yong Liang FENG
1
;
Su Ping WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Covalently closed circular deoxyribonucleic acid; Hepatitis B virus; Intrauterine transmission; Peripheral blood mononuclear cells; Serological markers
- MeSH: Adolescent; Adult; DNA, Viral; blood; Disease Transmission, Infectious; Female; Hepatitis B; congenital; transmission; Hepatitis B e Antigens; blood; Humans; Infant, Newborn; Leukocytes, Mononuclear; virology; Male; Middle Aged; Young Adult
- From: Biomedical and Environmental Sciences 2019;32(5):315-323
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the relationship between maternal peripheral blood mononuclear cells (PBMC) hepatitis B virus (HBV) covalenty closed circular deoxyribonucleic acid (cccDNA) and other HBV serological markers and its effects on HBV intrauterine transmission.
METHODS:We enrolled 290 newborns and their hepatitis B surface antigen (HBsAg) positive mothers. HBV cccDNA in PBMC and HBV DNA in serum were detected by a real-time PCR-TaqMan probe while HBV serological markers were detected with an electrochemiluminescence immunoassay.
RESULTS:There was a positive correlation between the levels of PBMC HBV cccDNA and serum HBV DNA and HBeAg (r = 0.436 and 0.403, P < 0.001). The detection rate of pattern A ['HBsAg (+), HBeAg (+), and anti-HBc (+)'] was significantly higher in the PBMC HBV cccDNA positive group than in the control group (χ2 = 48.48, P < 0.001). There was a significant association between HBV intrauterine transmission and PBMC HBV cccDNA (χ2 = 9.28, P = 0.002). In the presence of serum HBV DNA, HBeAg, and PBMC HBV cccDNA, the risk of HBV intrauterine transmission was three times higher (OR = 3.69, 95% CI: 1.30-10.42) than that observed in their absence. The risk of HBV intrauterine transmission was the greatest (OR = 5.89, 95% CI: 2.35-14.72) when both PBMC HBV cccDNA and pattern A were present. A Bayesian network model showed that maternal PBMC HBV cccDNA was directly related to HBV intrauterine transmission.
CONCLUSION:PBMC HBV cccDNA may be a direct risk factor for HBV intrauterine transmission. Our study suggests that serological markers could be combined with PBMC-related markers in prenatal testing.
