Dynamics of Proliferation and Differentiation after Transplantation of Hematopoietic Cells in Mouse.
10.19746/j.cnki.issn.1009-2137.2019.03.051
- Author:
Fang DONG
1
;
Sen ZHANG
1
;
Zi-Ning YANG
1
;
Ai GAO
1
;
Xiao-Fang WANG
1
;
Feng-Jiao WANG
1
;
Shi-Hui MA
1
;
Sha HAO
2
Author Information
1. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, State Center of Clinical Medical Research of Blood Diseases, Tianjin 300020, China.
2. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, State Center of Clinical Medical Research of Blood Diseases, Tianjin 300020, China,E-mail: haosha@ihcams.ac.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Transplantation;
Cell Differentiation;
Cell Proliferation;
Hematopoietic Stem Cell Transplantation;
Hematopoietic Stem Cells;
Mice;
Mice, Inbred C57BL
- From:
Journal of Experimental Hematology
2019;27(3):950-957
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the dynamic changes of hematopoietic reconstitution and multiple lineages differentiation at early phase after transplantation.
METHODS:Whole bone marrow mononuclear cells (wBMMNC, 5×10) and enriched c-Kit hematopoietic stem/progenitor cells (HSPC, 3×10) from the BM of B6-Ly5.1 mice were transplanted into lethally irradiated B6-Ly5.2 mice, the frequencies and absolute numbers of donor-derived cells (including LKS and LKS) were detected by flow cytometry. The multiple lineages differentiation of donor-derived cells was also monitored by flow cytometry. The homing and early phase proliferations of donor-derived cells were observed by two-photon microscope.
RESULTS:The donor-derived cells started to proliferation from 5-7 days after transplantation and reached the peak value at 2-3 weeks after wBMMNC transplantation. The donor-derived cells proliferated from 1-2 weeks and maintained until 4 weeks after c-kitHSPC transplantation. At 1 week after transplantation, the donor-derived cells mainly differentiated into myeloid cells with a few lymphoid cells production (B cells) but the production of T cells was not observed at most in wBMMNC transplanted group, while myeloid cells occupied the majority of donor-derived cells at 2-4 weeks; donor-derived cells almost totally differentiated into myeloid cells at 1-3 weeks after transplantation in c-Kit transplanted group and donor-derived B cells appeared at 4 weeks. The absolute number of donor-derived LKS and LKS cells in the BM of c-Kit transplanted group were much higher than that of wBMMNC group (P＜0.001) at 2 weeks respectively. The clustering proliferation of cKit cells at 4-5 days after transplantation was observed by two photon microscope.
CONCLUSION:The dynamical rate of proliferation and reconstitution of donor-derived cells are much earlier and quicker in c-Kit group than those of wBMMNC group. c-Kit cells mainly differentiate into myeloid cells within 1-3 weeks and the lymphoid cell differentiation starts at 4 weeks after transplantation. The immediate proliferation and differentiation of c-Kit cells within 1 week maybe due to the urgent needs of hematopoietic regeneration under the myeloablated hosts.
