Increment of N-cadherin Expression in Bone Marrow Mesenchymal Stem Cells Accompanies by Imparment of Bone Marrow B Lymphocyte Production in MRL/lpr Lupus Mice.
10.7534/j.issn.1009-2137.2019.03.053
- Author:
Li-Xia WANG
1
;
Hua LU
2
;
Xiao-Ming FEI
3
;
Yu TANG
4
;
Wei SHI
4
;
Ping LU
3
;
Yan ZHU
3
Author Information
1. Department of Hematology, The First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，Jiangsu Province, China; Department of Hematology, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China.
2. Department of Hematology, The First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，Jiangsu Province, China;E-mail:luhua1956@aliyun.com.
3. Department of Hematology, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China.
4. Department of Reumatology, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Animals;
B-Lymphocytes;
Bone Marrow;
Bone Marrow Cells;
Cadherins;
Mesenchymal Stem Cells;
Mice;
Mice, Inbred C57BL;
Mice, Inbred MRL lpr
- From:
Journal of Experimental Hematology
2019;27(3):964-969
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the hematological changes in MRL/lpr lupus mice and detect N-cadherin expression in their bone marrow mesenchymal stem cell (BMMSC).
METHODS:Peripheral blood cell count was analyzed. The ratio of each lineage in bone marrow was analyzed by flow cytometry. Bone marrow CFU-pre-B, BFU-E and CFU-GM were detected by colony formation assay. Expression of N-cadherin in BMMSC was analyzed by Western blot before and after treatment with the BMP/Smad pathway aganist BMP-2 and inhibitor Noggin.
RESULTS:Hemoglobin, red blood cell count and hematocrit decreased in lupus mice, compared with C57BL/6 mice. The ratio of B220+ lymphocyte and the number of CFU-pre-B in bone marrow of lupus mice decreased. Expression of N-cadherin in BMMSC of lupus mice was higher than that in the control group. Expression of N-cadherin decreased in BMP-2-treated BMMSC and increased after Noggin treatment.
CONCLUSION:Hematological changes in lupus mice include anemia and impairment of bone marrow B cell production. The expression of N-cadherin in BMMSC of lupus mice increases which maybe involved in abnormal hemogenesis in MRI/Ipr lupus mice.
