Multi-component quantitation of artificial cow-bezoar and study on its markers of quality difference.
10.19540/j.cnki.cjcmm.2018.0015
- Author:
Yan SHI
1
;
Dong-Mei SUN
2
;
Jing XIONG
1
;
Feng WEI
1
;
Shuang-Cheng MA
1
Author Information
1. National Institutes for Food and Drug Control, Beijing 100050, China.
2. Jilin Institute for Food and Drug Control, Jilin 132001, China.
- Publication Type:Journal Article
- Keywords:
ELSD;
HPLC;
artificial cow-bezoar;
bile acids;
chemometrics;
quality marker
- MeSH:
Animals;
Bezoars;
Cattle;
Chromatography, High Pressure Liquid;
Medicine, Chinese Traditional;
Quality Control
- From:
China Journal of Chinese Materia Medica
2018;43(4):659-664
- CountryChina
- Language:Chinese
-
Abstract:
An HPLC-ELSD method for quantitation of GCANa, CA, HDCA, CDCA and DCA in artificial cow-bezoar was developed in this study. The chromatographic separation was performed using a Wondasil CC₁₈(4.6 mmχ250 mm，5 μm) column at a column temperature of 25 °C and liquid flow-rate of 1.0 mL·min⁻¹. Acetonitrile and 0.2% formic acid solution were used as mobile phase with a linear gradient and injection volume was 10 μL. An ELSD was used with a nitrogen flow-rate of 2.3 L·h⁻¹at a drift tube temperature of 110 °C. Some chemometric methods were applied in data procession and analysis for exploration of the markers of quality difference. The accurate and simple method is suitable for the quality evaluation and the quality control of artificial cow-bezoar.
