Evaluation of the periodontal and pulpal healing of replanted rat molars with doxycycline root conditioning
10.5051/jpis.2019.49.3.148
- Author:
Ok Hyung NAM
1
;
Kyounga CHEON
;
Mi Sun KIM
;
Hyo Seol LEE
;
Sung Chul CHOI
Author Information
1. Department of Pediatric Dentistry, Kyung Hee University School of Dentistry, Seoul, Korea. pedochoi@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Doxycycline;
Regeneration;
Replantation;
Tooth injuries
- MeSH:
Animals;
Doxycycline;
Molar;
Rats;
Rats, Sprague-Dawley;
Regeneration;
Replantation;
Root Resorption;
Tooth;
Tooth Injuries
- From:Journal of Periodontal & Implant Science
2019;49(3):148-157
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study aimed to evaluate periodontal and pulpal healing in replanted rat teeth, preserved under different storage conditions, with or without root conditioning using doxycycline. METHODS: A total of 40 maxillary first molars extracted from 20 Sprague-Dawley rats were stored for different durations under different conditions (5 minutes in dry storage and 60 minutes in Hank's balanced salt solution [HBSS]) and subsequently replanted. The rats were divided into 2 groups based on the use of root surface treatment: the doxycycline group (root surface treated with doxycycline) and the control group (no doxycycline treatment). Eight weeks after replantation, the animals were sacrificed, and the teeth were evaluated using micro-computed tomography (micro-CT) and histomorphometric analysis. RESULTS: In the micro-CT analysis, the doxycycline group showed the same rate of occurrence of periapical radiolucency as was observed in the control group, but a lower degree of root resorption in teeth replanted after 60 minutes of storage in HBSS (P<0.05). In the histomorphometric analysis, the doxycycline group exhibited no improvement in either pulpal or periodontal healing of the replanted tooth after 5 minutes of dry storage, but showed a lower grade of surface root resorption (1.37±0.77) and inflammatory resorption in the teeth stored for 60 minutes in HBSS (1.33±0.71). CONCLUSIONS: In conclusion, doxycycline improved the periodontal healing of replanted teeth stored for 60 minutes in HBSS, whereas doxycycline did not improve periodontal healing of replanted tooth after 5 minutes of dry storage. Within the limits of this study, doxycycline showed more favorable periodontal healing despite delayed replantation.
