Prominent Inflammatory Features of Monocytes/Macrophages in Acute Calcium Pyrophosphate Crystal Arthritis: a Comparison with Acute Gouty Arthritis
- Author:
Ji Hye JEONG
1
;
Jae Hyung JUNG
;
Jung Sun LEE
;
Ji Seon OH
;
Yong Gil KIM
;
Chang Keun LEE
;
Bin YOO
;
Seokchan HONG
Author Information
- Publication Type:Brief Communication
- Keywords: Calcium pyrophosphate; Gout; Monocytes; Macrophages
- MeSH: Arthritis; Arthritis, Gouty; Calcium Pyrophosphate; Calcium; Cytokines; Gout; Humans; Interleukin-10; Interleukin-8; Macrophages; Monocytes; Phenotype; Phosphotransferases; Proto-Oncogenes; Synovial Fluid
- From:Immune Network 2019;19(3):e21-
- CountryRepublic of Korea
- Language:English
- Abstract: Calcium pyrophosphate (CPP) crystals can present as acute inflammatory arthritis which is known as an acute CPP crystal arthritis. Although monocytes/macrophages have been shown to play a role in the initiation of crystal-mediated inflammatory responses, differences in their phenotypes between acute CPP crystal arthritis and acute gouty arthritis have not yet been investigated. We examined the immunological characteristics of synovial monocytes/macrophages in patients with acute CPP crystal and acute gouty arthritis. CD14⁺CD3⁻CD19⁻CD56⁻ cell frequencies in synovial fluid mononuclear cells (SFMCs) were measured. Expression of pro- and anti-inflammatory cytokines and markers was determined. The SFMCs were dominated by a population of monocytes/macrophages in acute CPP crystal arthritis similar to that in acute gout. Synovial monocytes/macrophages showed the phenotypes of infiltrated monocytes as shown by expression of CD88, C-C chemokine receptor type 2, myeloid-related protein (MRP)8 and MRP14 but not proto-oncogene tyrosine-protein kinase MER. Comparatively, the CD14⁺ cells from patients with acute CPP crystal arthritis had similar high levels of IL-1β and TNF-α production but significantly lower expression of IL-10 and M2 marker (CD163). The monocytes/macrophages had the capacity to produce IL-8 in response to CPP crystals. Proinflammatory features were more dominant in monocytes/macrophages during acute CPP crystal arthritis than those during acute gouty arthritis.
