Effects of heme oxygenase-1 upregulation on isoproterenol-induced myocardial infarction
10.4196/kjpp.2019.23.3.203
- Author:
Somaia A G ELTOBSHY
1
;
Abdelaziz M HUSSEIN
;
Asaad A ELMILEEGY
;
Mona H ASKAR
;
Yomna KHATER
;
Emile F METIAS
;
Ghada M HELAL
Author Information
1. Department of Physiology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt. menhag@mans.edu.eg
- Publication Type:Original Article
- Keywords:
Connexin 43;
Heme oxygenase-1;
HSP70 heat-shock proteins;
Isoproterenol-myocardial infarction
- MeSH:
Adult;
Animals;
Antioxidants;
Body Weight;
Cobalt;
Connexin 43;
Connexins;
Creatine;
Electrocardiography;
Glutathione;
Hand;
Heart Rate;
Heat-Shock Proteins;
Heme Oxygenase-1;
Heme;
HSP70 Heat-Shock Proteins;
Humans;
Isoproterenol;
Male;
Muscles;
Myocardial Infarction;
Myocardium;
Oxidoreductases;
Rats;
Tromethamine;
Up-Regulation
- From:The Korean Journal of Physiology and Pharmacology
2019;23(3):203-217
- CountryRepublic of Korea
- Language:English
-
Abstract:
The present study was designed to examine the effect of heme oxygenase-1 (HO-1) induction by cobalt protoporphyrin (CoPP) on the cardiac functions and morphology, electrocardiogram (ECG) changes, myocardial antioxidants (superoxide dismutase [SOD] and glutathione [GSH]), and expression of heat shock protein (Hsp) 70 and connexin 43 (Cx-43) in myocardial muscles in isoproterenol (ISO) induced myocardial infarction (MI). Thirty two adult male Sprague Dawely rats were divided into 4 groups (each 8 rats): normal control (NC) group, ISO group: received ISO at dose of 150 mg/kg body weight intraperitoneally (i.p.) for 2 successive days; ISO + Trizma group: received (ISO) and Trizma (solvent of CoPP) at dose of 5 mg/kg i.p. injection 2 days before injection of ISO, with ISO at day 0 and at day 2 after ISO injections; and ISO + CoPP group: received ISO and CoPP at a dose of 5 mg/kg dissolved in Trizma i.p. injection as Trizma. We found that, administration of ISO caused significant increase in heart rate, corrected QT interval, ST segment, cardiac enzymes (lactate dehydrogenase, creatine kinase-muscle/brain), cardiac HO-1, Hsp70 with significant attenuation in myocardial GSH, SOD, and Cx-43. On the other hand, administration of CoPP caused significant improvement in ECG parameters, cardiac enzymes, cardiac morphology; antioxidants induced by ISO with significant increase in HO-1, Cx-43, and Hsp70 expression in myocardium. In conclusions, we concluded that induction of HO-1 by CoPP ameliorates ISO-induced myocardial injury, which might be due to up-regulation of Hsp70 and gap junction protein (Cx-43).
