Dioscorea japonica Thunb. Ethanolic Extract Attenuated Oxazolone-Induced Atopic Dermatitis-like Skin Lesions in BALB/c Mice
10.20307/nps.2019.25.3.261
- Author:
Jonghwan JEGAL
1
;
No June PARK
;
Beom Geun JO
;
Su Nam KIM
;
Min Hye YANG
Author Information
1. College of Pharmacy, Pusan National University, Busan 46241, Korea. mhyang@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Dioscorea japonica Thunb.;
Atopic dermatitis;
Oxazolone;
Interleukin 4
- MeSH:
Animals;
Dermatitis;
Dermatitis, Atopic;
Dexamethasone;
Dioscorea;
Ear;
Enzyme-Linked Immunosorbent Assay;
Eosine Yellowish-(YS);
Ethanol;
Hematoxylin;
Immunoglobulin E;
Interleukin-4;
Korea;
Mice;
Oxazolone;
Polyuria;
Rhizome;
Skin;
Therapeutic Uses;
Tolonium Chloride
- From:Natural Product Sciences
2019;25(3):261-267
- CountryRepublic of Korea
- Language:English
-
Abstract:
The rhizomes of Dioscorea japonica Thunb. are widely consumed as food and also used to treat diabetes and polyuria in Korea. This study was undertaken to study the anti-atopic dermatitis effects of a 95% ethanolic extract (DJE) of D. japonica in an oxazolone-stimulated murine model of atopic dermatitis (AD). The therapeutic effects of DJE on AD-like skin lesions were assessed on both ears. DJE (1%) or dexamethasone (0.5%; the positive control) were applied to skin lesions for three weeks. Serum levels of IgE and IL-4 were assessed by ELISA (enzyme-linked immunosorbent assay). Histopathological examinations were performed by hematoxylin and eosin (H&E) and toluidine blue staining and revealed DJE significantly reduced dermal thickness and inflammatory cell infiltration when applied to oxazolone-treated ear skin. DJE-treated AD mice also showed lower serum levels of IgE and IL-4 than oxazolone-stimulated controls. Our findings demonstrate DJE might be a useful safe, topical agent for the treatment of atopic diseases.
