Simultaneous Extraction and Separation of Oil and Azadirachtin from Seeds and Leaves of Azadirachta indica using Binary Solvent Extraction
10.20307/nps.2019.25.2.150
- Author:
Sheela SUBRAMANIAN
1
;
Aiza Syuhaniz SALLEH
;
Robert Thomas BACHMANN
;
Md. Sohrab HOSSAIN
Author Information
1. Branch Campus Malaysian Institute of Chemical and Bio-Engineering Technology, Green Chemistry & Sustainable Engineering Technology Cluster, Universiti Kuala Lumpur, 78000 Alor Gajah, Malacca, Malaysia. bachmann@unikl.edu.my
- Publication Type:Original Article
- Keywords:
Azadirachtin;
limonoids;
neem oil;
binary solvent extraction;
botanical insecticide
- MeSH:
Azadirachta;
Ethanol;
Limonins;
Solvents
- From:Natural Product Sciences
2019;25(2):150-156
- CountryRepublic of Korea
- Language:English
-
Abstract:
Conventional extraction of oil and azadirachtin, a botanical insecticide, from Azadirachta indica involves defatting the seeds and leaves using hexane followed by azadirachtin extraction with a polar solvent. In order to simplify the process while maintaining the yield we explored a binary extraction approach using Soxhlet extraction device and hexane and ethanol as non-polar and polar solvents at various ratios and extraction times. The highest oil and azadirachtin yields were obtained at 6 h extraction time using a 50:50 solvent mixture for both neem leaves (44.7 wt%, 720 mg(Aza)/kg(leaves)) and seeds (53.5 wt%, 1045 mg(Aza)/kg(seeds)), respectively.
