Expression of YB-1 enhances production of murine leukemia virus vectors by stabilizing genomic viral RNA.
10.1007/s13238-012-2090-x
- Author:
Wei LI
1
;
Xinlu WANG
;
Guangxia GAO
Author Information
1. Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Gene Expression;
Genetic Engineering;
methods;
Genetic Vectors;
biosynthesis;
genetics;
Genome, Viral;
genetics;
HEK293 Cells;
Humans;
Leukemia Virus, Murine;
genetics;
physiology;
RNA Stability;
genetics;
RNA, Viral;
chemistry;
genetics;
Repetitive Sequences, Nucleic Acid;
genetics;
Untranslated Regions;
genetics;
Virion;
genetics;
physiology;
Y-Box-Binding Protein 1;
genetics
- From:
Protein & Cell
2012;3(12):943-949
- CountryChina
- Language:English
-
Abstract:
Murine leukemia virus (MLV)-based retroviral vectors is widely used for gene transfer and basic research, and production of high-titer retroviral vectors is very important. Here we report that expression of the Y-box binding protein 1 (YB-1) enhanced the production of infectious MLV vectors. YB-1 specifically increased the stability of viral genomic RNA in virus-producing cells, and thus increasing viral RNA levels in both producer cells and virion particles. The viral element responsive to YB-1 was mapped to the repeat sequence (R region) in MLV genomic RNA. These results identified YB-1 as a MLV mRNA stabilizer, which can be used for improving production of MLV vectors.
