Hrs inhibits citron kinase-mediated HIV-1 budding via its FYVE domain.
10.1007/s13238-011-1053-y
- Author:
Jiwei DING
1
;
Lishan SU
;
Guangxia GAO
Author Information
1. Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing.
- Publication Type:Journal Article
- MeSH:
Down-Regulation;
Endosomal Sorting Complexes Required for Transport;
genetics;
metabolism;
Endosomes;
metabolism;
Exocytosis;
Gene Expression;
Gene Silencing;
drug effects;
HEK293 Cells;
HIV Infections;
genetics;
metabolism;
virology;
HIV-1;
drug effects;
genetics;
growth & development;
Humans;
Immunoprecipitation;
Intracellular Signaling Peptides and Proteins;
genetics;
metabolism;
Microscopy, Fluorescence;
Phosphoproteins;
genetics;
metabolism;
Plasmids;
Protein Binding;
drug effects;
genetics;
Protein Interaction Domains and Motifs;
Protein Structure, Tertiary;
Protein Transport;
Protein-Serine-Threonine Kinases;
genetics;
metabolism;
RNA, Small Interfering;
pharmacology;
Transfection;
Virion;
drug effects;
genetics;
growth & development;
Virus Release;
drug effects;
Virus Replication;
drug effects
- From:
Protein & Cell
2011;2(6):470-476
- CountryChina
- Language:English
-
Abstract:
Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a key component of the endosomal sorting complexes required for transport and has been demonstrated to play a regulatory role in endocytosis/exocytosis and the accumulation of internal vesicles in multivesicular bodies. Citron kinase is a Ser/The kinase that we previously reported to enhance human immunodeficiency virus type 1 (HIV-1) virion production. However, the relationship between Hrs and citron kinase in HIV-1 production remains elusive. Here, we report that Hrs interacts with citron kinase via its FYVE domain. Overexpression of Hrs or the FYVE domain resulted in a significant decrease in HIV-1 virion production. Depletion of Hrs by RNA interference in HEK293T cells increased HIV-1 virion production and enhanced the activity of citron kinase. These data suggest that Hrs inhibits HIV-1 production by inhibiting citron kinase-mediated exocytosis.
