Effects of total saponins of panax notoginseng on depressive behavior and hippocampal nerve regen-eration in rats with following global cerebral ischemia depression
10.3760/cma.j.issn.1674-6554.2019.06.002
- VernacularTitle:三七总皂苷对全脑缺血后抑郁大鼠抑郁行为及海马神经再生的影响
- Author:
Xu HE
1
;
Yan TANG
;
Zehua YANG
;
Fengjun DENG
Author Information
1. 益阳医学高等专科学校解剖教研室 413000;湖南中医药大学中西医结合学院
- Keywords:
Total saponins of panax notoginseng;
Hippocampus;
Nerve regeneration;
Follow-ing global cerebral ischemia depression;
Rats
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2019;28(6):487-492
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of the total saponins of panax notoginseng ( TSPN) on depression-like behavior following global cerebral ischemia depression in rats and its mechanism. Method-s Using four-vessel occlusion method to build the global cerebral ischemia model,then the cerebral ischemi-a rats were given solitary breeding with chronic unpredictable mild stress ( CUMS) to prepare depression model. Seventy rats were divided into sham group (n=10),model group ( n=20),PSD group ( n=20) and TSPN group (n=20). The rats in the TSPN group were administered TSPN intraperitoneally 30 min post-brain ischemia. The dose of TSPN (75mg/kg) was suspended in 0. 9% saline 10g/L,once per day for 30 days after reperfusion. While rats in the vehicle group and PSD group was treated with equal volume of 0. 9% saline,one injection per day until the rats were sacrificed at 30 days after brain ischemia. The BrdU,dou-blecortin (DCX) expression in the hippocampus was assessed by immunohistochemistry. Results In com-parison with the model group,the sucrose preference percentage in the PSD group was significantly lower ((46. 2±9. 2)%,(61. 2±7. 6)%;t=3. 18,P<0. 05),then the PSD rats were administered TSPN intraperito-neally,the sucrose preference percentage increased significantly ((62. 4±3. 4)%,(46. 2±9. 2)%;t=3. 43, P<0. 05). During the forced swimming test,the immobility time of PSD group was significantly increased compared with the model group ((119. 4±9. 7)s,(88. 0±15. 6)s ;t=4. 30,P<0. 01),while after PSD rats administering TSPN intraperitoneally,the immobility time was shorten remarkably ((97. 4±6. 7)s,(119. 4± 9. 7)s;t=3. 01,P<0. 05). Compared with the Model group( BrdU+:( 12. 6± 2. 2)/mm2,DCX+:( 38. 6± 4. 2)/mm2),the number of BrdU+ and DCX+ cells in the SGZ of hippocampus in PSD group decreased (BrdU+:(8. 8±1. 5)/mm2,DCX+:(27. 2±2. 8)/mm2;t=3. 25,4. 29,both P<0. 01). And compared with PSD group,the number of BrdU+ and DCX+ cells in the SGZ of hippocampus in TSPN group increased sig-nificantly (BrdU+:(14. 8±2. 8)/mm2,DCX+:(37. 0±3. 3)/mm2;t=4. 68,3. 69,both P<0. 05). Conclu-sion TSPN can improve the depression-like behavior of rats following global cerebral ischemia,which may be related with promoting hippocampal nerve regeneration.
