Intestinal fatty acid-binding protein expression in the intestinal tissue and clinical significance in neonatal rats with necrotizing enterocolitis
10.3760/cma.j.issn.2096-2932.2019.05.014
- VernacularTitle:肠型脂肪酸结合蛋白在新生大鼠坏死性小肠结肠炎肠道组织中的表达及意义
- Author:
Ying HE
1
;
Jinglin XU
;
Dongmei CHEN
;
Ruiquan WANG
;
Lianqiang WU
Author Information
1. 福建省泉州市儿童医院新生儿科 362000
- Keywords:
Enterocolitis;
necrotizing;
Fatty acid-binding proteins;
Rats
- From:Chinese Journal of Neonatology
2019;34(5):385-389
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of intestinal intestinal fatty acid-binding protein (I-FABP) and its clinical significance in an experimental model of necrotizing enterocolitis (NEC) in neonatal rats. Method Twenty-four neonatal Sprague-Dawley (SD) rats were randomly assigned into 4 groups at 48 h after birth (6 rats in each group):group A (control group), group B (NEC group-1), group C (NEC group-2), and group D (NEC group-3). The neonatal rats were fed by the mother rats in the same cage within 48 h after birth. After 48 h, the NEC group received artificial feeding, hypoxia, cold stimulation and lipopolysaccharide (LPS) gavage (10 mg/kg). NEC group-1, 2 and 3 were sacrificed on an empty stomach at 1, 2 and 3 d after the modeling. The control group was sacrificed on an empty stomach 3 d after the modeling without special treatment. Intestinal tissue were obtained from each rats. The histological changes of ileal tissues were studied using hematoxylin-eosin (HE) staining. The expressions of intestinal I-FABP were detected using RT-PCR and ELISA methods. Result Compared with the control group, body weight of rats in NEC group-1, 2 and 3 were lower, and pathology scores in these three groups were higher (P<0.05). The levels of intestinal I-FABP mRNA in NEC group-1, NEC group-2 and NEC group-3 were 2.69±0.27, 2.12±0.09, 3.18± 0.22, respectively. The protein expression levels were 363.7 ± 11.4, 321.7 ± 45.8, 432.3 ± 50.3, respectively. The mRNA and protein levels were all significantly higher than the control group (mRNA: 1.00 ± 0.02, protein: 134.2 ± 24.0, P<0.05). Conclusion I-FABP was a useful marker for ischemic injury to the intestine. These findings may contribute to a better diagnosis of NEC in newborns.
