Experimental study of periostin promoting rapid distraction osteogenesis of the rabbit mandible
10.12016/j.issn.2096-1456.2019.09.002
- Author:
JIANG Xiaowen
1
;
HUANG Huaqing
1
;
CHEN Jinyong
1
;
PENG Haiyan
1
Author Information
1. Department of Stomatology, the First People’s Hospital of Chenzhou City, the South Medical University & Institute of Translation Medicine, University of China South
- Publication Type:Journal Article
- Keywords:
periostin;
rabbit;
mandible;
distraction osteogenesis;
bone regeneration;
bone mineralization density;
bone mineral content
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2019;27(9):551-556
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the promoting effect of periostin on rapid distraction osteogenesis of the rabbit mandible and provide experimental evidence for the clinical use of periostin to promote osteogenesis.
Methods:Twenty-four New Zealand male white rabbits underwent distraction osteogenesis, and after 3 days of retention, they were rapidly stretched at a stretch rate of 2 mm/d (total 5 d). The animals were randomly divided into group A and group B (12 per group). On the last day of the stretch, 0.5 mL of normal saline containing 40 μg of recombinant periostin was given to group B or an equal volume of normal saline was added to the control group (group A) for 8 days. At 4 weeks and 8 weeks post-stretch, 8 animals were randomly selected from each group to undergo a CT scan under general anesthesia. The bone mineral density and bone mineral content were detected by dual energy X-ray absorptiometry. Eight weeks post-stretch, all of the experimental animals were sacrificed. Six animals were randomly selected from each group for micro-CT and a histological examination, and the remaining animals were subjected to biomechanical tests.
Results :CT images showed that the new bone formation in the distraction space of group B was significantly better than that of group A at 4 and 8 weeks post-stretch. At 4 weeks and 8 weeks post-stretch, the bone mineral density in group B was (0.157 ± 0.016) g/cm 2 and (0.234 ± 0.023) g/cm 2, respectively, and the bone mineral content was (0.096 ± 0.010) g and (0.204 ± 0.017) g, respectively. The above four means were significantly higher in group B than in group A (P < 0.001). The micro-CT images and data suggest that the stretch gap microstructure of group B has more mature features. Histological experiments showed that the trabecular bone of group B was thick and mature, with few chondrocytes. The biomechanical test results showed that the biomechanical strength of the distraction gap in group B was (228.47 ± 39.98) N, which was 1.24 times that of group A (P = 0.045).
Conclusion: Interstitial use of periosteal protein in the distraction space of the mandible in rabbits can promote local new bone formation and mineralization.
- Full text:骨膜蛋白促进兔下颌骨快速牵张成骨的实验研究1.pdf
