Inhibitory effect of NF-kappaB p65siRNA on human laryngeal carcinoma xenograft model in nude mice.
- Author:
Hongjun JIN
1
,
2
;
Weihua LOU
3
;
Jianzhong SANG
3
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, China. jinhongjun1@
2. com
3. Department of Otorhinolaryngology-Head and Neck Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Carcinoma, Squamous Cell;
genetics;
pathology;
Cell Line, Tumor;
Female;
Gene Expression Regulation, Neoplastic;
Humans;
Laryngeal Neoplasms;
genetics;
pathology;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
RNA, Small Interfering;
Transcription Factor RelA;
genetics;
Xenograft Model Antitumor Assays;
bcl-X Protein;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2013;27(15):836-838
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the inhibitory effect of NF-kappaB p65siRNA on human laryngeal carcinoma xenograft model in nude mice.
METHOD:Human laryngeal carcinoma cell line Hep-2 was seeded in the subcutaneous layer of 15 nude mice to build laryngeal carcinoma xenograft model. Then they were randomly divided into three groups. NF-kappaB p65siRNA was given in siRNA group and FAM-Control siRNA was given in negative control group while phosphoric-buffered saline (PBS) was used in normal control group for 3 weeks. Tumor size and body weight of the mice were measured. TUNEL method and immunohistochemical S-P method were used for detecting the expression of NF-kappaB p65 and Bcl-xL protein.
RESULT:The volume of tumors in siRNA group was reduced and the average weight of tumors in siRNA group was lower than the other two groups (P < 0.05). In siRNA group, the expression of NF-kappaB p65 and Bcl-xL protein was down-regulated and the apoptotic rate was increased obviously as compared with the negative control group and the normal control group.
CONCLUSION:NF-kappaB p65siRNA can significantly inhibit the expression of NF-kappaB p65 and the growth of human laryngeal carcinoma xenograft in nude mice. Its mechanism may be related to inducing the apoptosis in tumor cells by down-regulating the expression of Bcl-xL protein.
