Effects of c-Met-siRNA on the biological behaviour of laryngeal carcinoma Hep-2 cells.
- Author:
Zhinian XIE
1
;
Changyou JI
;
Jichuan CHEN
;
Yi'nan WANG
;
Liqian GUAN
;
Hongtao LI
;
Min ZHANG
;
Junhui YANG
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Daping Hospital, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
genetics;
Carcinoma, Squamous Cell;
genetics;
pathology;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Laryngeal Neoplasms;
genetics;
pathology;
Liposomes;
Proto-Oncogene Proteins c-met;
genetics;
RNA, Messenger;
genetics;
RNA, Small Interfering;
genetics;
Transfection
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2009;23(12):553-560
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effects of c-Met-siRNA on the proliferation, movement and invasion of laryngeal carcinoma Hep-2 cells in vitro.
METHOD:Firstly, the pSilencer 2.0/c-Met-shRNA recombinant plasmid was transfected into laryngeal carcinoma Hep-2 cells with transfecting agent of cationic liposome Lipofectamine 2000. Secondly,the transfection efficacy was tested by RT-PCR and Western-Blot, then the most inhibitive c-Met-siRNA sequence was elected. Cell proliferation, movement and invasion were detected with MTT, cell migration assay and cell invasion assay, respectively.
RESULT:After the transfection of pSilencer 2.0/c-Met-shRNA recombinant plasmid into laryngeal carcinoma Hep-2 cells, the expression of mRNA and protein of c-Met decreased significantly in Hep-2 cells, and ability of the proliferation, movement and invasion of laryngeal carcinoma Hep-2 cells were also inhibited.
CONCLUSION:The results indicated that c-Met-siRNA can down-regulated the expression of c-Met and markedly inhibited laryngeal carcinoma Hep-2 cell proliferation, movement and invasion. It may have the potential as a therapeutic modality to treat human laryngeal carcinoma.
