The inhibitory effect of FA-MNP-MMP-9-ASODN nanocomposite on nasopharyngeal carcinoma in vitro.
- Author:
Tao LIU
;
Minqiang XIE
;
Yiming XU
;
Hongzheng ZHANG
;
Tao ZHANG
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Carcinoma;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Humans;
Matrix Metalloproteinase 9;
metabolism;
Nanocomposites;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
metabolism;
pathology;
Transfection
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(9):607-611
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the inhibitory effect of FA-MNP-MMP-9-ASODN nanocomposite on nasopharyngeal carcinoma (NPC) HNE-1 cell in vitro.
METHOD:To observe the MMP-9 mRNA and protein expression levels, proliferation ability, cell apoptosis and invasion ability of HNE-1 cell 48 hours after FA-MNP-MMP-9-ASODN transfection by RT-PCR, Western-blot, MTT assay, flow cytometry and Matrigel invasion test.
RESULT:MMP-9 mRNA and protein expression in HNE-1 cell of FA-MNP-MMP-9-ASODN group was significantly decreased compared to control group and nonsense sequence group of FA-MNP-MMP-9-NSODN. At the same time, for the HNE-1 cell in FA-MNP-MMP-9-ASODN group, growth inhibition rate was about 35.66%, proliferation activity significantly decreased compared to the control group and the nonsense sequence group, cell cycle was also inhibited, cell apoptosis rate was about 12.60%, the apoptosis rate was significantly higher than that in the control group and the nonsense sequence group. Invasion assay showed that the transmembrane cells in FA-MNP-MMP-9-ASODN group were about 21.00, significantly lower than that in the control group and the nonsense sequence group.
CONCLUSION:By inhibiting the expression of MMP-9, FA-MNP-MMP-9-ASODN nanocomposites could reduce NPC cell proliferation and invasion ability, and promote apoptosis, it had a good inhibitory effect in vitro.
