Construction and expression of the targeting super-antigen EGF-SEA fusion gene.
- Author:
Yang XIE
;
Shaoping PENG
;
Zhiying LIAO
;
Jiafeng LIU
;
Xuemei LIU
;
Weifeng CHEN
- Publication Type:Journal Article
- MeSH:
Enterotoxins;
genetics;
Epidermal Growth Factor;
genetics;
Genetic Vectors;
Head and Neck Neoplasms;
drug therapy;
Humans;
Molecular Targeted Therapy;
Recombinant Fusion Proteins;
biosynthesis;
genetics
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(9):638-656
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To construct expression vector for the SEA-EGF fusion gene.
METHOD:Clone the SEA gene and the EGF gene segment with PCR and RT-PCR independently, and connect this two genes by the bridge PCR. Insert the fusion gene EGF-SEA into the expression vector PET-44. Induced the secretion of the fusion protein SEA-EGF by the antileptic.
RESULT:The gene fragment encoding EGF and SEA mature peptide was successfully cloned. The fusion gene EGF-SEA was successfully constructed and was inserted into expression vector.
CONCLUSION:The new recombinant expression vector for fusion gene EGF-SEA is specific for head and neck cancer, laid the foundation for the further study of fusion protein SEA-EGF targeting immune therapy in head and neck tumors.
